Biohydrogen production using sheep manure hydrolysate as substrate shows promising application prospects, but the main bottleneck in developing this technology lies in the low reducing sugar content in the hydrolysate. Optimizing hydrolysis process parameters provides an effective solution to this problem, with the acid-enzyme hydrolysis method demonstrating significant advantages in sheep manure hydrolysis. This study systematically optimized the process parameters for acid-enzyme hydrolysis of sheep manure, including acid concentration, hydrolysis temperature, hydrolysis duration, enzymatic shaking speed, and enzyme ratio (cellulase to hemicellulase ratio). The optimal hydrolysis conditions were determined as: 8.0% hydrochloric acid concentration, hydrolysis temperature of 105°C, hydrolysis duration of 15 minutes, enzymatic shaking speed of 120 rpm, and 1:1 enzyme ratio (12 g/L cellulase + 12 g/L hemicellulase). Under these optimized conditions, the sheep manure hydrolysis rate reached 50.25±3.66%, with the reducing sugar content in hydrolysate increasing to 43.53±0.60 g/L. The research findings not only provide important references for optimizing sheep manure hydrolysis.

Sheep Manure, Acid-Enzyme Hydrolysis, Condition Optimization