Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation

Vivian Alves Pereira Silva; Gilson Teles Boaventura; Renato Souza Abboud; José Antonio Silva Ribas; Mauricio Alves Chagas

doi:doi:10.11648/j.ajss.20190704.12

Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation

Vivian Alves Pereira Silva, Gilson Teles Boaventura, Renato Souza Abboud, José Antonio Silva Ribas, Mauricio Alves Chagas

Published in American Journal of Sports Science (Volume 7, Issue 4)

Received: 29 July 2019 Accepted: 27 September 2019 Published: 10 October 2019

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Abstract

The abuse of anabolic androgenic steroids by youth and adolescents, athletes and non-athletes, is related to the occurrence of cardiovascular diseases. Contrastingly, green tea prevents and attenuates the cellular damage, improving the biochemical profile and reducing inflammation. This study aims to evaluate the benefits of consuming green tea to attenuate the inflammatory parameters and the cardiovascular damage, which are caused due to supraphysiological doses of testosterone, by analyzing the serological profile and the histoarchitecture of the heart and the arteries of 28 42-day-old male Wistar rats. Silicone pellets containing testosterone were surgically implanted and replaced every four weeks. After 20 weeks, all the male rats were anesthetized and their blood samples collected for serological analysis. Fragments of the aorta, left cardiac ventricle and penis were excised and processed to paraffin. To access morphometrical and stereological parameters, tissue fragments were stained and the images analyzed employing Image J software. The rats consuming green tea exhibited a reduction in aorta wall thickness (22%), luminal area (40%) and wall area (52%), with a lower number of elastic lamella. The dorsal artery of the penis presented with a higher wall area and lower luminal area in induced group compared to the other three groups. Tea consumption also led to a significant reduction in the left ventricle thickness with no increase in volumetric density of collagen fibers. Inflammatory cytokines were also shown to have increased in induced group, indicating cardiovascular risk due to their exposure to testosterone. Except for the endothelial vascular growth factor, this effect was not observed in the groups that consumed tea. It can be concluded that the consumption of green tea has a protective effect on the morphology of cardiovascular tissues and reduces inflammatory indicators, which points out this food as an agent capable of reducing cardiovascular disease risk factors related to high doses of anabolic androgenic steroids.

Published in	American Journal of Sports Science (Volume 7, Issue 4)
DOI	10.11648/j.ajss.20190704.12
Page(s)	141-148
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cardiovascular Diseases, Testosterone, Green Tea, Morphometry, Cytokines

References

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Vivian Alves Pereira Silva, Gilson Teles Boaventura, Renato Souza Abboud, José Antonio Silva Ribas, Mauricio Alves Chagas. (2019). Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. American Journal of Sports Science, 7(4), 141-148. https://doi.org/10.11648/j.ajss.20190704.12

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Vivian Alves Pereira Silva; Gilson Teles Boaventura; Renato Souza Abboud; José Antonio Silva Ribas; Mauricio Alves Chagas. Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. Am. J. Sports Sci. 2019, 7(4), 141-148. doi: 10.11648/j.ajss.20190704.12

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AMA Style

Vivian Alves Pereira Silva, Gilson Teles Boaventura, Renato Souza Abboud, José Antonio Silva Ribas, Mauricio Alves Chagas. Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. Am J Sports Sci. 2019;7(4):141-148. doi: 10.11648/j.ajss.20190704.12

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@article{10.11648/j.ajss.20190704.12,
  author = {Vivian Alves Pereira Silva and Gilson Teles Boaventura and Renato Souza Abboud and José Antonio Silva Ribas and Mauricio Alves Chagas},
  title = {Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation},
  journal = {American Journal of Sports Science},
  volume = {7},
  number = {4},
  pages = {141-148},
  doi = {10.11648/j.ajss.20190704.12},
  url = {https://doi.org/10.11648/j.ajss.20190704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajss.20190704.12},
  abstract = {The abuse of anabolic androgenic steroids by youth and adolescents, athletes and non-athletes, is related to the occurrence of cardiovascular diseases. Contrastingly, green tea prevents and attenuates the cellular damage, improving the biochemical profile and reducing inflammation. This study aims to evaluate the benefits of consuming green tea to attenuate the inflammatory parameters and the cardiovascular damage, which are caused due to supraphysiological doses of testosterone, by analyzing the serological profile and the histoarchitecture of the heart and the arteries of 28 42-day-old male Wistar rats. Silicone pellets containing testosterone were surgically implanted and replaced every four weeks. After 20 weeks, all the male rats were anesthetized and their blood samples collected for serological analysis. Fragments of the aorta, left cardiac ventricle and penis were excised and processed to paraffin. To access morphometrical and stereological parameters, tissue fragments were stained and the images analyzed employing Image J software. The rats consuming green tea exhibited a reduction in aorta wall thickness (22%), luminal area (40%) and wall area (52%), with a lower number of elastic lamella. The dorsal artery of the penis presented with a higher wall area and lower luminal area in induced group compared to the other three groups. Tea consumption also led to a significant reduction in the left ventricle thickness with no increase in volumetric density of collagen fibers. Inflammatory cytokines were also shown to have increased in induced group, indicating cardiovascular risk due to their exposure to testosterone. Except for the endothelial vascular growth factor, this effect was not observed in the groups that consumed tea. It can be concluded that the consumption of green tea has a protective effect on the morphology of cardiovascular tissues and reduces inflammatory indicators, which points out this food as an agent capable of reducing cardiovascular disease risk factors related to high doses of anabolic androgenic steroids.},
 year = {2019}
}

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TY  - JOUR
T1  - Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation
AU  - Vivian Alves Pereira Silva
AU  - Gilson Teles Boaventura
AU  - Renato Souza Abboud
AU  - José Antonio Silva Ribas
AU  - Mauricio Alves Chagas
Y1  - 2019/10/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ajss.20190704.12
DO  - 10.11648/j.ajss.20190704.12
T2  - American Journal of Sports Science
JF  - American Journal of Sports Science
JO  - American Journal of Sports Science
SP  - 141
EP  - 148
PB  - Science Publishing Group
SN  - 2330-8540
UR  - https://doi.org/10.11648/j.ajss.20190704.12
AB  - The abuse of anabolic androgenic steroids by youth and adolescents, athletes and non-athletes, is related to the occurrence of cardiovascular diseases. Contrastingly, green tea prevents and attenuates the cellular damage, improving the biochemical profile and reducing inflammation. This study aims to evaluate the benefits of consuming green tea to attenuate the inflammatory parameters and the cardiovascular damage, which are caused due to supraphysiological doses of testosterone, by analyzing the serological profile and the histoarchitecture of the heart and the arteries of 28 42-day-old male Wistar rats. Silicone pellets containing testosterone were surgically implanted and replaced every four weeks. After 20 weeks, all the male rats were anesthetized and their blood samples collected for serological analysis. Fragments of the aorta, left cardiac ventricle and penis were excised and processed to paraffin. To access morphometrical and stereological parameters, tissue fragments were stained and the images analyzed employing Image J software. The rats consuming green tea exhibited a reduction in aorta wall thickness (22%), luminal area (40%) and wall area (52%), with a lower number of elastic lamella. The dorsal artery of the penis presented with a higher wall area and lower luminal area in induced group compared to the other three groups. Tea consumption also led to a significant reduction in the left ventricle thickness with no increase in volumetric density of collagen fibers. Inflammatory cytokines were also shown to have increased in induced group, indicating cardiovascular risk due to their exposure to testosterone. Except for the endothelial vascular growth factor, this effect was not observed in the groups that consumed tea. It can be concluded that the consumption of green tea has a protective effect on the morphology of cardiovascular tissues and reduces inflammatory indicators, which points out this food as an agent capable of reducing cardiovascular disease risk factors related to high doses of anabolic androgenic steroids.
VL  - 7
IS  - 4
ER  -

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Author Information

Vivian Alves Pereira Silva

Biomedical Institute, Federal Fluminense University, Niteroi, Brazil
Gilson Teles Boaventura

Nutrition Faculty, Federal Fluminense University, Niteroi, Brazil
Renato Souza Abboud

Biomedical Institute, Federal Fluminense University, Niteroi, Brazil
José Antonio Silva Ribas

Department of Physiology and Pharmacology, Federal Fluminense University, Niteroi, Brazil
Mauricio Alves Chagas

Biomedical Institute, Federal Fluminense University, Niteroi, Brazil

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Vivian Alves Pereira Silva, Gilson Teles Boaventura, Renato Souza Abboud, José Antonio Silva Ribas, Mauricio Alves Chagas. (2019). Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. American Journal of Sports Science, 7(4), 141-148. https://doi.org/10.11648/j.ajss.20190704.12

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Vivian Alves Pereira Silva; Gilson Teles Boaventura; Renato Souza Abboud; José Antonio Silva Ribas; Mauricio Alves Chagas. Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. Am. J. Sports Sci. 2019, 7(4), 141-148. doi: 10.11648/j.ajss.20190704.12

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AMA Style

Vivian Alves Pereira Silva, Gilson Teles Boaventura, Renato Souza Abboud, José Antonio Silva Ribas, Mauricio Alves Chagas. Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation. Am J Sports Sci. 2019;7(4):141-148. doi: 10.11648/j.ajss.20190704.12

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@article{10.11648/j.ajss.20190704.12,
  author = {Vivian Alves Pereira Silva and Gilson Teles Boaventura and Renato Souza Abboud and José Antonio Silva Ribas and Mauricio Alves Chagas},
  title = {Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation},
  journal = {American Journal of Sports Science},
  volume = {7},
  number = {4},
  pages = {141-148},
  doi = {10.11648/j.ajss.20190704.12},
  url = {https://doi.org/10.11648/j.ajss.20190704.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajss.20190704.12},
  abstract = {The abuse of anabolic androgenic steroids by youth and adolescents, athletes and non-athletes, is related to the occurrence of cardiovascular diseases. Contrastingly, green tea prevents and attenuates the cellular damage, improving the biochemical profile and reducing inflammation. This study aims to evaluate the benefits of consuming green tea to attenuate the inflammatory parameters and the cardiovascular damage, which are caused due to supraphysiological doses of testosterone, by analyzing the serological profile and the histoarchitecture of the heart and the arteries of 28 42-day-old male Wistar rats. Silicone pellets containing testosterone were surgically implanted and replaced every four weeks. After 20 weeks, all the male rats were anesthetized and their blood samples collected for serological analysis. Fragments of the aorta, left cardiac ventricle and penis were excised and processed to paraffin. To access morphometrical and stereological parameters, tissue fragments were stained and the images analyzed employing Image J software. The rats consuming green tea exhibited a reduction in aorta wall thickness (22%), luminal area (40%) and wall area (52%), with a lower number of elastic lamella. The dorsal artery of the penis presented with a higher wall area and lower luminal area in induced group compared to the other three groups. Tea consumption also led to a significant reduction in the left ventricle thickness with no increase in volumetric density of collagen fibers. Inflammatory cytokines were also shown to have increased in induced group, indicating cardiovascular risk due to their exposure to testosterone. Except for the endothelial vascular growth factor, this effect was not observed in the groups that consumed tea. It can be concluded that the consumption of green tea has a protective effect on the morphology of cardiovascular tissues and reduces inflammatory indicators, which points out this food as an agent capable of reducing cardiovascular disease risk factors related to high doses of anabolic androgenic steroids.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Green Tea Consumption Lower Damage and Inflammatory Cytokines in Rat Cardiovascular System Under Androgenic Stimulation
AU  - Vivian Alves Pereira Silva
AU  - Gilson Teles Boaventura
AU  - Renato Souza Abboud
AU  - José Antonio Silva Ribas
AU  - Mauricio Alves Chagas
Y1  - 2019/10/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ajss.20190704.12
DO  - 10.11648/j.ajss.20190704.12
T2  - American Journal of Sports Science
JF  - American Journal of Sports Science
JO  - American Journal of Sports Science
SP  - 141
EP  - 148
PB  - Science Publishing Group
SN  - 2330-8540
UR  - https://doi.org/10.11648/j.ajss.20190704.12
AB  - The abuse of anabolic androgenic steroids by youth and adolescents, athletes and non-athletes, is related to the occurrence of cardiovascular diseases. Contrastingly, green tea prevents and attenuates the cellular damage, improving the biochemical profile and reducing inflammation. This study aims to evaluate the benefits of consuming green tea to attenuate the inflammatory parameters and the cardiovascular damage, which are caused due to supraphysiological doses of testosterone, by analyzing the serological profile and the histoarchitecture of the heart and the arteries of 28 42-day-old male Wistar rats. Silicone pellets containing testosterone were surgically implanted and replaced every four weeks. After 20 weeks, all the male rats were anesthetized and their blood samples collected for serological analysis. Fragments of the aorta, left cardiac ventricle and penis were excised and processed to paraffin. To access morphometrical and stereological parameters, tissue fragments were stained and the images analyzed employing Image J software. The rats consuming green tea exhibited a reduction in aorta wall thickness (22%), luminal area (40%) and wall area (52%), with a lower number of elastic lamella. The dorsal artery of the penis presented with a higher wall area and lower luminal area in induced group compared to the other three groups. Tea consumption also led to a significant reduction in the left ventricle thickness with no increase in volumetric density of collagen fibers. Inflammatory cytokines were also shown to have increased in induced group, indicating cardiovascular risk due to their exposure to testosterone. Except for the endothelial vascular growth factor, this effect was not observed in the groups that consumed tea. It can be concluded that the consumption of green tea has a protective effect on the morphology of cardiovascular tissues and reduces inflammatory indicators, which points out this food as an agent capable of reducing cardiovascular disease risk factors related to high doses of anabolic androgenic steroids.
VL  - 7
IS  - 4
ER  -

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