Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plated on selective and differential media. Isolation and identification of bacteria were carried out using standard microbiological method, Antibiotics susceptibility test was determined by the disk diffusion method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were carried out by standard method. Eight bacteria were selected; Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp. Escherichia coli, Shigella sp. and Klebsiella pneumonia, they were resistant to at least seven antibiotics. The result revealed that the plasmid size ranged from 8.60 to 128.00 kilobase pair (kbp) and the number of plasmid carried by bacteria ranged from 1 to 3 plasmids. Based on the 16s rRNA sequences, the following bacteria were identified Salmonella enterica subsp.enterica serovar Typhi str. CT18, Proteus mirabilis strain HI4320, Pseudomonas fluorescens SBW25, Staphylococcus cohnii subsp. cohnii strain 532 Contig16, Salmonella enterica subsp. enterica serovar Infantis, Escherichia coli str. K12 substr. DH10B, Shigella flexneri 2a str. 2457T and Klebsiella pneumoniae strain J1. The presence of multiple resistant bacteria and plasmid mediated resistance of human pathogenic bacteria isolated from poultry droppings in Akure is of great public health importance.
| Published in | Biochemistry and Molecular Biology (Volume 2, Issue 1) |
| DOI | 10.11648/j.bmb.20170201.12 |
| Page(s) | 6-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Bacteria, Molecular Identification, Antibiotics, Plasmid
| [1] | Adzitey, F. and Nurul, H. (2011). Campylobacter in poultry: incidences and possible control measures. Research Journal of Microbiology. 6: 182–192. |
| [2] | Adzitey, F., Huda, N. and Gulam, R. (2011). Comparison of media for the isolation of Salmonella (XLD and Rambach) and Listeria species (ALOA and Palcam) in naturally contaminated duck samples. International Journal of Food Safety. 13: 20–25. |
| [3] | Apata, D. F. (2009). Antibiotic Resistance in Poultry. International Journal of Poultry Science. 8 (4): 404-408. |
| [4] | Collignon, P. (2009). Resistant Escherichia coli – We Are What We Eat, Clinical Infectious Diseases, 49: 202-204. |
| [5] | Hammerum, A. M. and Heuer, O. E., (2009). Human health hazards from antimicrobial-resistant Escherichia coli of animal origin, Clinical Infectious Diseases, 48: 916-921. |
| [6] | Nahar, A., Siddiquee, M., Nahar, S., Anwar, K. S. and Islam, S. (2014). Multidrug Resistant-Proteus Mirabilis Isolated from Chicken Droppings in Commercial Poultry Farms: Bio-security Concern and Emerging Public Health Threat in Bangladesh. Journal of Biosafety and Health Education. 2: 120-128. |
| [7] | Brindha, V. and Ani, A. M. (2012). Molecular characterization and identification of unknown bacteria from waste water. Indian Journal of Innovations and Development. 1(2): 87-91. |
| [8] | Magistrado, P., Carcia, M. and Raymundo, A. (2001). Isolation and polymerase chain reaction-base detection of Campylobacter jejuni and Campylobacter coli from poultry in Philippines. International Journal of Food Microbiology. 70: 194–206. |
| [9] | Keramas, G., Bang, D. D., Lund, M., Madsen, M., Bunkenborg, H., Telleman, P. and Christensen, C. B. V. (2004). Use of culture, PCR analysis and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken faeces. Journal of Clinical Microbiology. 47: 3985-3991. |
| [10] | Okeke, I. N., Fayinka, S. T. and Lamikanra, A. (2000). Antibiotic resistance in Escherichia coli from Nigerian students, 1986 1998. Emerging Infectious Disease. 6: 393–396. |
| [11] | Zhang, R., Eggleston, K., Rotimi, V., and Zeckhauser, R. J. (2006). Antibiotic resistance as a global threat: evidence from China, Kuwait and the United States. Global Health 2: 61-69. |
| [12] | Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries. 2nd Edition., Cambridge University Press, Cambridge, UK., ISBN-13:9781139449298. 50: 165-176. |
| [13] | Committee for Clinical Laboratory Standards, (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24 Wayne, PA: ISBN 1-56238-898-3. 34(1): 50-98. |
| [14] | Liu, P. Y., Lau, Y. J. and Hu, B. S. (1995). Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods. Journal of Clinical Microbiology, 33: 1779-1783. |
| [15] | Nicole, J. M., Zhilong, G. and Xing-fung, L. I. (2004). Reverse Transcription- multiplex PCR Assay for Simultaneous Detection of E. coli O157:H7, Vibrio cholera O1 and Salmonella typhi. Journal of Clinical Chemistry, 50: 2037-2044. |
| [16] | Kariuki, S., Gilks, C., Kimari, J., Obanda, A., Muyodi J., Waiyaki, P. and Hart, C. (1999). Genotype analysis of E. coli strains from children and chicken living in close contact, Applied and Environmental microbiology, 65(2): 472-476. |
| [17] | Parkhill, J., Dougan, K. D., James, N. R., Thomas, D., Pickard, J., Wain, C., Churcher, K. L., Mungall, S. D., Bentley, M. T. G., Holden, M. et al., (2001). Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature 413: 848-852. |
| [18] | Khan, A. U. and Musharraf, A. (2004). Plasmid-mediated multiple antibiotic resistance in Proteus mirabilis isolated from patients with urinary tract infection. Medical Science Monitoring. 10(11): 598-602. |
| [19] | Nsofor, C. A. and Iroegbu, C. U. (2013). Plasmid profile of antibiotic resistant Escherichia coli isolated from domestic animals in South-East Nigeria. Journal of Cell and Animal Biology. 7(9):109-115. |
| [20] | Ajayi, A. O. and Egbebi, A. O. (2011). Antibiotic sucseptibility of Salmonella Typhi and Klebsiella Pneumoniae from poultry and local birds in Ado-Ekiti, Ekiti-State, Nigeria. Annals of Biological Research. 2 (3):431-437. |
| [21] | Gundogan, N. and Avci, E. (2013). Prevalence and antibiotic resistance of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey. African Journal of Microbiology Research. 7(31), 4059-4064. |
| [22] | Clarridge III, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology. 17: 840-862. |
| [23] | Woo, P. C. Y., Lau, S. K. P., Teng, J. L. L., Tse, H., and Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical Microbiology and Infection. 14: 908-934. |
| [24] | Harris, K. A., Fidler, K. J., Hartley, J. C., Vogt, J., Klein, N. J., Monsell, F. and Novelli, V. M. (2002). Unique case of Helicobacter sp. osteomyelitis in an immunocompetent child diagnosed by broad-range 16S PCR. Journal of Clinical Microbiology. 40: 3100-3103. |
| [25] | Frickmann, H., Dekker, D., Schwarz, N. G., Hahn, A., Boahen, K., Sarpong, N., Adu-Sarkodie, Y., Halbgewachs, H., Marks, F., von Kalckreuth, V., May, J. and Hagen, R. M. (2015). 16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa. PLOS ONE | DOI:10.1371/journal.pone.0135923: 1-20. |
APA Style
Kehinde Oluyemi Ajayi, Funmilola Oluyemi Omoya. (2017). Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Biochemistry and Molecular Biology, 2(1), 6-11. https://doi.org/10.11648/j.bmb.20170201.12
ACS Style
Kehinde Oluyemi Ajayi; Funmilola Oluyemi Omoya. Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Biochem. Mol. Biol. 2017, 2(1), 6-11. doi: 10.11648/j.bmb.20170201.12
AMA Style
Kehinde Oluyemi Ajayi, Funmilola Oluyemi Omoya. Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Biochem Mol Biol. 2017;2(1):6-11. doi: 10.11648/j.bmb.20170201.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.bmb.20170201.12,
author = {Kehinde Oluyemi Ajayi and Funmilola Oluyemi Omoya},
title = {Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria},
journal = {Biochemistry and Molecular Biology},
volume = {2},
number = {1},
pages = {6-11},
doi = {10.11648/j.bmb.20170201.12},
url = {https://doi.org/10.11648/j.bmb.20170201.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170201.12},
abstract = {Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plated on selective and differential media. Isolation and identification of bacteria were carried out using standard microbiological method, Antibiotics susceptibility test was determined by the disk diffusion method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were carried out by standard method. Eight bacteria were selected; Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp. Escherichia coli, Shigella sp. and Klebsiella pneumonia, they were resistant to at least seven antibiotics. The result revealed that the plasmid size ranged from 8.60 to 128.00 kilobase pair (kbp) and the number of plasmid carried by bacteria ranged from 1 to 3 plasmids. Based on the 16s rRNA sequences, the following bacteria were identified Salmonella enterica subsp.enterica serovar Typhi str. CT18, Proteus mirabilis strain HI4320, Pseudomonas fluorescens SBW25, Staphylococcus cohnii subsp. cohnii strain 532 Contig16, Salmonella enterica subsp. enterica serovar Infantis, Escherichia coli str. K12 substr. DH10B, Shigella flexneri 2a str. 2457T and Klebsiella pneumoniae strain J1. The presence of multiple resistant bacteria and plasmid mediated resistance of human pathogenic bacteria isolated from poultry droppings in Akure is of great public health importance.},
year = {2017}
}
TY - JOUR T1 - Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria AU - Kehinde Oluyemi Ajayi AU - Funmilola Oluyemi Omoya Y1 - 2017/03/22 PY - 2017 N1 - https://doi.org/10.11648/j.bmb.20170201.12 DO - 10.11648/j.bmb.20170201.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 6 EP - 11 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20170201.12 AB - Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plated on selective and differential media. Isolation and identification of bacteria were carried out using standard microbiological method, Antibiotics susceptibility test was determined by the disk diffusion method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were carried out by standard method. Eight bacteria were selected; Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp. Escherichia coli, Shigella sp. and Klebsiella pneumonia, they were resistant to at least seven antibiotics. The result revealed that the plasmid size ranged from 8.60 to 128.00 kilobase pair (kbp) and the number of plasmid carried by bacteria ranged from 1 to 3 plasmids. Based on the 16s rRNA sequences, the following bacteria were identified Salmonella enterica subsp.enterica serovar Typhi str. CT18, Proteus mirabilis strain HI4320, Pseudomonas fluorescens SBW25, Staphylococcus cohnii subsp. cohnii strain 532 Contig16, Salmonella enterica subsp. enterica serovar Infantis, Escherichia coli str. K12 substr. DH10B, Shigella flexneri 2a str. 2457T and Klebsiella pneumoniae strain J1. The presence of multiple resistant bacteria and plasmid mediated resistance of human pathogenic bacteria isolated from poultry droppings in Akure is of great public health importance. VL - 2 IS - 1 ER -
Information
Email: