Distinct Role of Transforming Growth Factor-Beta 1 and Fibroblast Growth Factors in Human Amelobastoma Epithelial Cell Proliferation
Yuko Matsuda,
Naoko Kamogashira,
Yuji Hatakeyama,
Toshinari Mikami,
Kazuki Nakashima,
Junko Hatakeyama,
Sachio Tamaoki,
Yoshihiko Sawa,
Hiroyuki Ishikawa
Issue:
Volume 2, Issue 1, January 2017
Pages:
1-5
Received:
30 January 2017
Accepted:
22 February 2017
Published:
9 March 2017
Abstract: Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the development of the enamel organ reports that FGF-9, -10, and TGF-β1 are strongly involved in dental epithelial cell differentiation and cell proliferation. In this study, we attempt to evaluate the effect of these growth factors on ameloblastoma cells. Both collagen-coated and normal plastic cell culture plate cell growth curves were steeper in the presence of growth supplement than in the absence of growth supplement. The presence of TGF-β1 at each dose (1 to 10 ng/ml), however, suppressed the number of cells cultured on the collagen-coated plate but made no significant difference on the normal plastic plate. The number of cells was increased in the presence of FGF-10 at 100 ng/ml, but not in the presence of FGF-9 after 48 h culture. These results suggest that FGF-10 and TGF-β1 play distinct roles in the cell proliferation of human ameloblastoma cells.
Abstract: Ameloblastoma is a locally invasive benign epithelial odontogenic tumor and its histopathological structures are similar to the enamel organ. Although various studies have investigated cell proliferation in ameloblastoma to elucidate the biological behavior and clinicopathological mechanisms, it remains poorly understood. The studies on the develop...
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Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria
Kehinde Oluyemi Ajayi,
Funmilola Oluyemi Omoya
Issue:
Volume 2, Issue 1, January 2017
Pages:
6-11
Received:
27 February 2017
Accepted:
8 March 2017
Published:
22 March 2017
Abstract: Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plated on selective and differential media. Isolation and identification of bacteria were carried out using standard microbiological method, Antibiotics susceptibility test was determined by the disk diffusion method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were carried out by standard method. Eight bacteria were selected; Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp. Escherichia coli, Shigella sp. and Klebsiella pneumonia, they were resistant to at least seven antibiotics. The result revealed that the plasmid size ranged from 8.60 to 128.00 kilobase pair (kbp) and the number of plasmid carried by bacteria ranged from 1 to 3 plasmids. Based on the 16s rRNA sequences, the following bacteria were identified Salmonella enterica subsp.enterica serovar Typhi str. CT18, Proteus mirabilis strain HI4320, Pseudomonas fluorescens SBW25, Staphylococcus cohnii subsp. cohnii strain 532 Contig16, Salmonella enterica subsp. enterica serovar Infantis, Escherichia coli str. K12 substr. DH10B, Shigella flexneri 2a str. 2457T and Klebsiella pneumoniae strain J1. The presence of multiple resistant bacteria and plasmid mediated resistance of human pathogenic bacteria isolated from poultry droppings in Akure is of great public health importance.
Abstract: Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plate...
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