Abstract: Antibiotic resistance has been growing at an alarming rate and consequently the activity of antibiotics has dropped dramatically day by day. Metal complex [Co (SB) (SCN)] (where, SB = 2-[(6-Amino-hexylimino)-methyl] phenol) have been reported for the purpose of searching new antimicrobial agents. The Schiff base and it’s transition metal complex was characterized by means of several physiochemical and spectroscopic methods. Several biomedical toxicological properties of the complex has been determined by monitoring the parameters cell growth inhibition, survival time of tumour mice, time-body relation, causing of intraperitoneal cells and macrophages, alkaline phosphatase activity, haematological effect and biopsy of tumour. The synthesized Schiff base Co (II) complex was found to have anticancer and cytotoxic function.Abstract: Antibiotic resistance has been growing at an alarming rate and consequently the activity of antibiotics has dropped dramatically day by day. Metal complex [Co (SB) (SCN)] (where, SB = 2-[(6-Amino-hexylimino)-methyl] phenol) have been reported for the purpose of searching new antimicrobial agents. The Schiff base and it’s transition metal complex wa...Show More
Abstract: In response to stress conditions such as wounding or infections in insects, several short peptides are processed to act as cytokines that induce AMP gene expression. To study their structure-activity relationship, immune inducibility, tissue specificity, stress responsiveness, and development relatedness, we chemically synthesized Manduca sexta stress response peptide-1, a 25-residue peptide with one disulfide bond (SRP1: FGVRVGTCPSGYVRRGTFCFPDDDY). Upon injection of the SRP1 into naïve larvae, several antimicrobial peptide genes were expressed at higher levels. The mRNA levels of SRP1 increased significantly in hemocytes and fat body after larvae were challenged with a mixture of bacteria and β-1,3-glucan. The expression patterns of SRP1 and its target genes are somewhat different from SRP2’s, suggesting overlapping yet distinct functions. We elucidated the 3D structure of SRP1 in solution by two-dimensional 1H-1H NMR spectroscopy. The tertiary structure of SRP1 consists of two short β-strands at Y12−R15 and F18−F20, one type-II β-turn at R15−F18 in its well-defined core and is stabilized by a covalent disulfide bond between C8 and C19. The conformational ensemble of SRP1 from extensive atomistic simulation in explicit solvent (with 3.0μs total effective sampling) shows high consistency with experimental intramolecular NOEs of the core region. The SRP1 core adopts a fold similar to the carboxyl-terminal subdomain of epidermal growth factor (EGF), suggesting that SRP1 may interact with EGF receptor-like molecules to trigger its biological function.Abstract: In response to stress conditions such as wounding or infections in insects, several short peptides are processed to act as cytokines that induce AMP gene expression. To study their structure-activity relationship, immune inducibility, tissue specificity, stress responsiveness, and development relatedness, we chemically synthesized Manduca sexta str...Show More
