Abstract: Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from broiler chickens were identified by measuring the dimensions of the sporulated oocyst. Seven species of Eimeria were detected using this method. When DNA was extracted and species specific primers were used to amplify ITS1 gene using single specific primer PCR and multiplex PCR, only six species were identified. The accuracy of identification of broiler chicken Eimeria species using PCR is more reliable than the conventional methods like öocyst measurements or histopathology of the affected intestinal regions. This is indicated by the finding that E. brunetti which was identified morphologically by öocyst measurement, could not be identified molecularly. The mitochondrial genome sequences (ITS1) are highly suited for molecular diagnostics of coccidia and may be a potential genetic marker for molecular epidemiology of broiler chicken coccidiosis in the future in Sudan. The aim of this study is to determine and identify the species causing poultry coccidiosis in broiler chicken by traditional methods and molecular characterization using ITS.Abstract: Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from bro...Show More
Abstract: Tuberculosis (TB) remains a major global health problem. In 2016, an estimated 10.4 million people developed TB and 1.8 million died from the disease. TB epidemiology in Cameroon has so far largely consisted of reporting the number of cases detected and their demographic data. The present study investigates the genetic diversity among Mycobacterium tuberculosis complex circulating in the west region of Cameroon and analyzes the relationship between genotypes and epidemiology situation of patient. Spoligotyping was performed by PCR-amplification followed by the reverse hybridization of 120 cultured specimens and were identified by comparison to reference strains in SITVIT database. Mycobacterium tuberculosis was the only species identified. LAM10_CAM and T1 families were the most prevalent genetic families with 43.33% and 31.66% respectively. No association was found between genotypes with HIV serostatus. However, there was a significant association between genotypes and the gender also between genotypes and Patients age. Patients with age above 35 were more likely infected by LAM10_CAM strains, and belong genotypes and locality where the patients of Dschang were more likely infected by T1 strain. Population structure of Mycobacterium tuberculosis complex strains from the West region of Cameroon was found to be diverse and the Share Type 61 from the LAM10_CAM family was highly predominant.Abstract: Tuberculosis (TB) remains a major global health problem. In 2016, an estimated 10.4 million people developed TB and 1.8 million died from the disease. TB epidemiology in Cameroon has so far largely consisted of reporting the number of cases detected and their demographic data. The present study investigates the genetic diversity among Mycobacterium...Show More
