Tuberculosis (TB) remains a major global health problem. In 2016, an estimated 10.4 million people developed TB and 1.8 million died from the disease. TB epidemiology in Cameroon has so far largely consisted of reporting the number of cases detected and their demographic data. The present study investigates the genetic diversity among Mycobacterium tuberculosis complex circulating in the west region of Cameroon and analyzes the relationship between genotypes and epidemiology situation of patient. Spoligotyping was performed by PCR-amplification followed by the reverse hybridization of 120 cultured specimens and were identified by comparison to reference strains in SITVIT database. Mycobacterium tuberculosis was the only species identified. LAM10_CAM and T1 families were the most prevalent genetic families with 43.33% and 31.66% respectively. No association was found between genotypes with HIV serostatus. However, there was a significant association between genotypes and the gender also between genotypes and Patients age. Patients with age above 35 were more likely infected by LAM10_CAM strains, and belong genotypes and locality where the patients of Dschang were more likely infected by T1 strain. Population structure of Mycobacterium tuberculosis complex strains from the West region of Cameroon was found to be diverse and the Share Type 61 from the LAM10_CAM family was highly predominant.
| Published in | Biochemistry and Molecular Biology (Volume 5, Issue 1) |
| DOI | 10.11648/j.bmb.20200501.12 |
| Page(s) | 6-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2020. Published by Science Publishing Group |
Tuberculosis, Spoligotypage, Cameroon, Genetic Diversity
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APA Style
Assam Assam Jean Paul, Feutsa Tegiogni Vanessa Therance, Godwe Celestin, Doungmo Toumbou Severin, Teuma Djeumetio Sylvain, et al. (2020). Genetic Diversity Using Spoligotyping Method in the Case of Pulmonary Tuberculosis in Certain Localities of West Region of Cameroon. Biochemistry and Molecular Biology, 5(1), 6-11. https://doi.org/10.11648/j.bmb.20200501.12
ACS Style
Assam Assam Jean Paul; Feutsa Tegiogni Vanessa Therance; Godwe Celestin; Doungmo Toumbou Severin; Teuma Djeumetio Sylvain, et al. Genetic Diversity Using Spoligotyping Method in the Case of Pulmonary Tuberculosis in Certain Localities of West Region of Cameroon. Biochem. Mol. Biol. 2020, 5(1), 6-11. doi: 10.11648/j.bmb.20200501.12
AMA Style
Assam Assam Jean Paul, Feutsa Tegiogni Vanessa Therance, Godwe Celestin, Doungmo Toumbou Severin, Teuma Djeumetio Sylvain, et al. Genetic Diversity Using Spoligotyping Method in the Case of Pulmonary Tuberculosis in Certain Localities of West Region of Cameroon. Biochem Mol Biol. 2020;5(1):6-11. doi: 10.11648/j.bmb.20200501.12
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Download@article{10.11648/j.bmb.20200501.12,
author = {Assam Assam Jean Paul and Feutsa Tegiogni Vanessa Therance and Godwe Celestin and Doungmo Toumbou Severin and Teuma Djeumetio Sylvain and Penlap Beng Veronique},
title = {Genetic Diversity Using Spoligotyping Method in the Case of Pulmonary Tuberculosis in Certain Localities of West Region of Cameroon},
journal = {Biochemistry and Molecular Biology},
volume = {5},
number = {1},
pages = {6-11},
doi = {10.11648/j.bmb.20200501.12},
url = {https://doi.org/10.11648/j.bmb.20200501.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20200501.12},
abstract = {Tuberculosis (TB) remains a major global health problem. In 2016, an estimated 10.4 million people developed TB and 1.8 million died from the disease. TB epidemiology in Cameroon has so far largely consisted of reporting the number of cases detected and their demographic data. The present study investigates the genetic diversity among Mycobacterium tuberculosis complex circulating in the west region of Cameroon and analyzes the relationship between genotypes and epidemiology situation of patient. Spoligotyping was performed by PCR-amplification followed by the reverse hybridization of 120 cultured specimens and were identified by comparison to reference strains in SITVIT database. Mycobacterium tuberculosis was the only species identified. LAM10_CAM and T1 families were the most prevalent genetic families with 43.33% and 31.66% respectively. No association was found between genotypes with HIV serostatus. However, there was a significant association between genotypes and the gender also between genotypes and Patients age. Patients with age above 35 were more likely infected by LAM10_CAM strains, and belong genotypes and locality where the patients of Dschang were more likely infected by T1 strain. Population structure of Mycobacterium tuberculosis complex strains from the West region of Cameroon was found to be diverse and the Share Type 61 from the LAM10_CAM family was highly predominant.},
year = {2020}
}
TY - JOUR T1 - Genetic Diversity Using Spoligotyping Method in the Case of Pulmonary Tuberculosis in Certain Localities of West Region of Cameroon AU - Assam Assam Jean Paul AU - Feutsa Tegiogni Vanessa Therance AU - Godwe Celestin AU - Doungmo Toumbou Severin AU - Teuma Djeumetio Sylvain AU - Penlap Beng Veronique Y1 - 2020/04/01 PY - 2020 N1 - https://doi.org/10.11648/j.bmb.20200501.12 DO - 10.11648/j.bmb.20200501.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 6 EP - 11 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20200501.12 AB - Tuberculosis (TB) remains a major global health problem. In 2016, an estimated 10.4 million people developed TB and 1.8 million died from the disease. TB epidemiology in Cameroon has so far largely consisted of reporting the number of cases detected and their demographic data. The present study investigates the genetic diversity among Mycobacterium tuberculosis complex circulating in the west region of Cameroon and analyzes the relationship between genotypes and epidemiology situation of patient. Spoligotyping was performed by PCR-amplification followed by the reverse hybridization of 120 cultured specimens and were identified by comparison to reference strains in SITVIT database. Mycobacterium tuberculosis was the only species identified. LAM10_CAM and T1 families were the most prevalent genetic families with 43.33% and 31.66% respectively. No association was found between genotypes with HIV serostatus. However, there was a significant association between genotypes and the gender also between genotypes and Patients age. Patients with age above 35 were more likely infected by LAM10_CAM strains, and belong genotypes and locality where the patients of Dschang were more likely infected by T1 strain. Population structure of Mycobacterium tuberculosis complex strains from the West region of Cameroon was found to be diverse and the Share Type 61 from the LAM10_CAM family was highly predominant. VL - 5 IS - 1 ER -
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