Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from broiler chickens were identified by measuring the dimensions of the sporulated oocyst. Seven species of Eimeria were detected using this method. When DNA was extracted and species specific primers were used to amplify ITS1 gene using single specific primer PCR and multiplex PCR, only six species were identified. The accuracy of identification of broiler chicken Eimeria species using PCR is more reliable than the conventional methods like öocyst measurements or histopathology of the affected intestinal regions. This is indicated by the finding that E. brunetti which was identified morphologically by öocyst measurement, could not be identified molecularly. The mitochondrial genome sequences (ITS1) are highly suited for molecular diagnostics of coccidia and may be a potential genetic marker for molecular epidemiology of broiler chicken coccidiosis in the future in Sudan. The aim of this study is to determine and identify the species causing poultry coccidiosis in broiler chicken by traditional methods and molecular characterization using ITS.
| Published in | Biochemistry and Molecular Biology (Volume 5, Issue 1) |
| DOI | 10.11648/j.bmb.20200501.11 |
| Page(s) | 1-5 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Apicomplexa, Broiler Chicken, Eimeria, ITS1, Poultry Coccidiosis
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APA Style
Mona Abdelrahman Mohamed Khaier, Hanan Ahmed Daffala, Abuelgasim Ibrahim Abdelhalim, Sumaia Mohamed Ahmed Abukashawa. (2020). Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene. Biochemistry and Molecular Biology, 5(1), 1-5. https://doi.org/10.11648/j.bmb.20200501.11
ACS Style
Mona Abdelrahman Mohamed Khaier; Hanan Ahmed Daffala; Abuelgasim Ibrahim Abdelhalim; Sumaia Mohamed Ahmed Abukashawa. Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene. Biochem. Mol. Biol. 2020, 5(1), 1-5. doi: 10.11648/j.bmb.20200501.11
AMA Style
Mona Abdelrahman Mohamed Khaier, Hanan Ahmed Daffala, Abuelgasim Ibrahim Abdelhalim, Sumaia Mohamed Ahmed Abukashawa. Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene. Biochem Mol Biol. 2020;5(1):1-5. doi: 10.11648/j.bmb.20200501.11
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Download@article{10.11648/j.bmb.20200501.11,
author = {Mona Abdelrahman Mohamed Khaier and Hanan Ahmed Daffala and Abuelgasim Ibrahim Abdelhalim and Sumaia Mohamed Ahmed Abukashawa},
title = {Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene},
journal = {Biochemistry and Molecular Biology},
volume = {5},
number = {1},
pages = {1-5},
doi = {10.11648/j.bmb.20200501.11},
url = {https://doi.org/10.11648/j.bmb.20200501.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20200501.11},
abstract = {Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from broiler chickens were identified by measuring the dimensions of the sporulated oocyst. Seven species of Eimeria were detected using this method. When DNA was extracted and species specific primers were used to amplify ITS1 gene using single specific primer PCR and multiplex PCR, only six species were identified. The accuracy of identification of broiler chicken Eimeria species using PCR is more reliable than the conventional methods like öocyst measurements or histopathology of the affected intestinal regions. This is indicated by the finding that E. brunetti which was identified morphologically by öocyst measurement, could not be identified molecularly. The mitochondrial genome sequences (ITS1) are highly suited for molecular diagnostics of coccidia and may be a potential genetic marker for molecular epidemiology of broiler chicken coccidiosis in the future in Sudan. The aim of this study is to determine and identify the species causing poultry coccidiosis in broiler chicken by traditional methods and molecular characterization using ITS.},
year = {2020}
}
TY - JOUR T1 - Isolation, Identification and Molecular Characterization of Eimeria spp Infecting Chicken in Khartoum State, Sudan Using ITS1 Gene AU - Mona Abdelrahman Mohamed Khaier AU - Hanan Ahmed Daffala AU - Abuelgasim Ibrahim Abdelhalim AU - Sumaia Mohamed Ahmed Abukashawa Y1 - 2020/03/17 PY - 2020 N1 - https://doi.org/10.11648/j.bmb.20200501.11 DO - 10.11648/j.bmb.20200501.11 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 1 EP - 5 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20200501.11 AB - Coccidiosis in broiler chickens highly affects the economy for both producers and consumers. The later get a low quality meat due to anaemia induced by the parasite. The disease is characterized by lesions caused by seven host specific members of the family Eimeriidae of the phylum Apicomplexa. Collected positive samples of Eimeria species from broiler chickens were identified by measuring the dimensions of the sporulated oocyst. Seven species of Eimeria were detected using this method. When DNA was extracted and species specific primers were used to amplify ITS1 gene using single specific primer PCR and multiplex PCR, only six species were identified. The accuracy of identification of broiler chicken Eimeria species using PCR is more reliable than the conventional methods like öocyst measurements or histopathology of the affected intestinal regions. This is indicated by the finding that E. brunetti which was identified morphologically by öocyst measurement, could not be identified molecularly. The mitochondrial genome sequences (ITS1) are highly suited for molecular diagnostics of coccidia and may be a potential genetic marker for molecular epidemiology of broiler chicken coccidiosis in the future in Sudan. The aim of this study is to determine and identify the species causing poultry coccidiosis in broiler chicken by traditional methods and molecular characterization using ITS. VL - 5 IS - 1 ER -
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