Constitutive Expression of <i>VvAATP</i> Increases Starch Content in Transgenic <i>Arabidopsis</i>

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang; Gaolei Ren; Bowen Wang; Qing Zhou; Xinhong Chen

doi:doi:10.11648/j.bmb.20170206.15

| Peer-Reviewed

Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, Gaolei Ren, Bowen Wang, Qing Zhou, Xinhong Chen

Published in Biochemistry and Molecular Biology (Volume 2, Issue 6)

Received: 5 September 2017 Accepted: 18 October 2017 Published: 22 November 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.

Published in	Biochemistry and Molecular Biology (Volume 2, Issue 6)
DOI	10.11648/j.bmb.20170206.15
Page(s)	94-101
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Arabidopsis, Constitutive Expression, Grapevine, Starch Content, VvAATP

References

[1]	Sanz-Barrio, R, Corral-Martinez, P, Ancin, M, et al. Overexpression of plastidial thioredoxin f leads to enhanced starch accumulation in tobacco leaves, Plant Biotechnol J. 2013; 11: 618-627.
[2]	Smith, AM. Prospects for increasing starch and sucrose yields for bioethanol production, Plant J. 2008; 54: 546-558.
[3]	Blennow, A, Jensen, SL, Shaik, SS, et al. Future cereal starch bioengineering cereal ancestors encounter gene technology and designer enzymes, Cereal Chem. 2013; 90: 274-287.
[4]	Skryhan, K, Cuesta-Seijo, JA, Nielsen, MM, et al. The role of cysteine residues in redox regulation and protein stability of Arabidopsis thaliana starch synthase 1, PLoS One. 2015; 10: e0136997.
[5]	Delvallé, D, Dumez, S, Wattebled, F, et al. Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves, Plant J. 2005; 43: 398-412.
[6]	Fujita, N, Yoshida, M, Asakura, N, et al. Function and characterization of starch synthase I using mutants in rice, Plant Physiol. 2006; 140: 1070-1084.
[7]	Jeon, JS, Ryoo, N, Hahn, TR, et al. Starch biosynthesis in cereal endosperm, Plant Physiol Bioch, 2010; 48: 383-392.
[8]	Fiore, C, Trézéguet, V, Saux, AL, et al. The mitochondrial ADP/ATP carrier: Structural, physiological and pathological aspects, Biochimie. 1998; 80: 137-150.
[9]	Winkler, HH, Neuhaus, HE. Non-mitochondrial ATP transport, Trends Biochem Sci. 1999; 24: 64-68.
[10]	Heldt, HW. Adenine nucleotide translocation in spinach chloroplasts, FEBS Lett. 1969; 5: 11-14.
[11]	Schünemann, D, Borchert, S, Flügge, UI, et al. ADP/ATP translocator from pea root plastids-Comparison with translocators from spinach chloroplasts and pea leaf mitochondria, Plant Physiol. 1993; 103: 131-137.
[12]	Emes, MJ, Neuhaus, HE. Metabolism and transport in non-photosynthetic plastids, J Exp Bot. 1997; 48: 1995-2005.
[13]	Möhlmann, T, Tjaden, J, Schwöppe, C, et al. Occurrence of two plastidic ATP/ADP transporters in Arabidopsis thaliana L-Molecular characterization and comparative structural analysis of similar ATP/ADP translocators from plastids and Rickettsia prowazekii, Eur J Biochem. 1998; 252: 353-359.
[14]	Linka, N, Hurka, H, Lang, BF, et al. Phylogenetic relationships of non-mitochondrial nucleotide transport proteins in bacteria and eukaryotes, Gene. 2003; 306: 27-35.
[15]	Meng K, Chang TJ, Liu X, et al. Cloning and expression pattern of a gene encoding a putative plastidic ATP/ADP transporter from Helianthus tuberosus L., J Integr Plant Biol. 2005; 47: 1123-1132.
[16]	Yuen CYL, Leelapon O, Chanvivattana Y, et al. Molecular characterization of two genes encoding plastidic ATP/ADP transport proteins in cassava, Biol Plantarum. 2009; 53: 37-44.
[17]	Tjaden J, Möhlmann T, Kampfenkel K, et al. Altered plastidic ATP/ADP-transporter activity influences potato (Solanum tuberosum L.) tuber morphology, yield and composition of tuber starch, Plant J. 1998; 16: 531-540.
[18]	Geigenberger P, Stamme C, Tjaden J, et al. Tuber physiology and properties of starch from tubers of transgenic potato plants with altered plastidic adenylate transporter activity, Plant Physiol. 2001; 125: 1667-1678.
[19]	Wang, FB, Ye, YX, Niu, Y, et al. A tomato plastidic ATP/ADP transporter gene SlAATP increases starch content in transgenic Arabidopsis, Physiol Mol Biol Plants. 2016; 22: 497-506.
[20]	Wang, FB. Fu, LF, Kong, WL, et al. Constitutive expression of StAATP, a potato plastidic ATP/ADP transporter gene, increases starch content in transgenic Arabidopsis, Biotechnol Biotec Eq. 2017; 31(2): 250-258.
[21]	Wang, FB, Chen, XH, Zhang, Fan, et al. A soybean plastidic ATP/ADP transporter gene GmAATP increases starch content in transgenic Arabidopsis, Plant Biotechnol Rep. 2017; 11: 135-146.
[22]	Wang, YN, Li, Y, Zhang, H, et al. A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose content and alters starch structure in transgenic sweetpotato, J Integr Agr. 2016; 15: 1968-1982.
[23]	Lou, XM, Yao, QH, Zhang, Z, et al. Expression of human hepatitis B virus large surface antigen gene in transgenic tomato, Clin Vaccine Immunol. 2007; 14: 464-469.
[24]	Zhang, X, Henriques, R, Lin, SS. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method, Nat Protoc. 2006; 1: 641-646.
[25]	Murashige, T, Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant. 1962; 15: 473-497.
[26]	Smith, AM, Zeeman, SC. Quantification of starch in plant tissues, Nat Protoc. 2006; 1: 1342-1345.
[27]	Li X, Ma H, Huang H, et al. Natural anthocyanins from phytoresources and their chemical researches, Nat Prod Res. 2013; 27: 456-469.
[28]	Schmittgen, TD, Livak, KJ. Analyzing real-time PCR data by the comparative C_T method, Nat Protoc. 2008; 3: 1101-1108.
[29]	Nakamura, Y, Yuki, K, Park, SY, et al. Carbohydrate metabolism in the developing endosperm of rice grains, Plant Cell Physiol. 1989; 30: 833-839.
[30]	Harrison, CJ, Mould, RM, Leech, MJ, et al. The rug3 locus of pea encodes plastidial phosphoglucomutase, Plant Physiol. 2000; 122: 1187-1192.
[31]	Jiang, T, Zhai, H, Wang, FB, et al. Cloning and characterization of a carbohydrate metabolism-associated gene IbSnRK1 from sweetpotato, Sci Hortic. 2013; 158: 22-32.
[32]	Wang, FB, Guo, XT, Qiao, XQ, et al. The maize plastidic thioredoxin F-type gene ZmTrxF increases starch accumulation in transgenic Arabidopsis, Sci Hortic. 2016; 210: 205-212.
[33]	Wang, FB, Kong, WL, Niu, Y, et al. StTrxF, a potato plastidic thioredoxin F-type protein gene, is involved in starch accumulation in transgenic Arabidopsis thaliana, Biotechnol Biotec Eq. 2017; 31 (3): 486-492.
[34]	Wang, FB, Kong, WL, Fu, YR, et al. Constitutive expression of SlTrxF increases starch content in transgenic Arabidopsis, Biol Plant. 2017; 61(3): 494-500.
[35]	Szydlowski, N, Ragel, P, Raynaud, S, et al. Starch granule initiation in Arabidopsis requires the presence of either class IV or class III starch synthase, Plant Cell. 2009; 21: 2443-2457.
[36]	Burton, RA, Jenner, H, Carrangis, L, et al. Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity, Plant J. 2002; 31: 97-112.
[37]	Bustos, R, Fahy, B, Hylton, CM, et al. Starch granule initiation is controlled by a heteromultimeric isoamylase in potato tubers, PNAS. 2004; 101: 2215-2220.
[38]	Roldan, I, Wattebled, F, Lucas, MM, et al. The phenotype of soluble starch synthase IV defective mutants of Arabidopsis thaliana suggests a novel function of elongation enzymes in the control of starch granule formation, Plant J. 2007; 49: 492-504.

Cite This Article

Plain Text BibTeX RIS

APA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. (2017). Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochemistry and Molecular Biology, 2(6), 94-101. https://doi.org/10.11648/j.bmb.20170206.15

Copy | Download

ACS Style

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem. Mol. Biol. 2017, 2(6), 94-101. doi: 10.11648/j.bmb.20170206.15

Copy | Download

AMA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem Mol Biol. 2017;2(6):94-101. doi: 10.11648/j.bmb.20170206.15

Copy | Download

@article{10.11648/j.bmb.20170206.15,
  author = {Feibing Wang and Bin Weng and Qi Wang and Yuxiu Ye and Jing Zhang and Gaolei Ren and Bowen Wang and Qing Zhou and Xinhong Chen},
  title = {Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {94-101},
  doi = {10.11648/j.bmb.20170206.15},
  url = {https://doi.org/10.11648/j.bmb.20170206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.15},
  abstract = {A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis
AU  - Feibing Wang
AU  - Bin Weng
AU  - Qi Wang
AU  - Yuxiu Ye
AU  - Jing Zhang
AU  - Gaolei Ren
AU  - Bowen Wang
AU  - Qing Zhou
AU  - Xinhong Chen
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.15
DO  - 10.11648/j.bmb.20170206.15
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.15
AB  - A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.
VL  - 2
IS  - 6
ER  -

Copy | Download

Author Information

Feibing Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bin Weng

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qi Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Yuxiu Ye

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Jing Zhang

School of Chemical Engineering, Huaiyin Institute of Technology, Huai’an, China
Gaolei Ren

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Bowen Wang

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Qing Zhou

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China
Xinhong Chen

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. (2017). Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochemistry and Molecular Biology, 2(6), 94-101. https://doi.org/10.11648/j.bmb.20170206.15

Copy | Download

ACS Style

Feibing Wang; Bin Weng; Qi Wang; Yuxiu Ye; Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem. Mol. Biol. 2017, 2(6), 94-101. doi: 10.11648/j.bmb.20170206.15

Copy | Download

AMA Style

Feibing Wang, Bin Weng, Qi Wang, Yuxiu Ye, Jing Zhang, et al. Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis. Biochem Mol Biol. 2017;2(6):94-101. doi: 10.11648/j.bmb.20170206.15

Copy | Download

@article{10.11648/j.bmb.20170206.15,
  author = {Feibing Wang and Bin Weng and Qi Wang and Yuxiu Ye and Jing Zhang and Gaolei Ren and Bowen Wang and Qing Zhou and Xinhong Chen},
  title = {Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {94-101},
  doi = {10.11648/j.bmb.20170206.15},
  url = {https://doi.org/10.11648/j.bmb.20170206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.15},
  abstract = {A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Constitutive Expression of VvAATP Increases Starch Content in Transgenic Arabidopsis
AU  - Feibing Wang
AU  - Bin Weng
AU  - Qi Wang
AU  - Yuxiu Ye
AU  - Jing Zhang
AU  - Gaolei Ren
AU  - Bowen Wang
AU  - Qing Zhou
AU  - Xinhong Chen
Y1  - 2017/11/22
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.15
DO  - 10.11648/j.bmb.20170206.15
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 94
EP  - 101
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.15
AB  - A plastidic ATP/ADP transporter (AATP) plays a crucial role in importing ATP from the cytosol into plastids, leading to the increase the ATP supply to facilitate anabolic synthesis in heterotrophic plastids of dicotyledonous plants. The regulatory role of the grapevine VvAATP gene in increasing starch accumulation has not been investigated. In this study, the VvAATP gene was successfully isolated from grapevine and transformed into Arabidopsis. Constitutive expression of VvAATP significantly increased starch accumulation in transgenic Arabidopsis plants. Real-time quantitative PCR analysis showed that constitutive expression of VvAATP up-regulated the expression of the genes related to starch biosynthesis pathway, including phosphoglucomutase, ADP-glucose pyrophosphorylase (AGPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) genes, in transgenic Arabidopsis plants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These results indicate that VvAATP may improve starch content of Arabidopsis by up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. All these findings suggest that the VvAATP gene may be applied for increasing starch accumulation of plants in the future.
VL  - 2
IS  - 6
ER  -

Copy | Download