Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers

El-Kawokgy Tahany Mohamed Abou El-Fath; Ibrahim Shafik Darwish; Ashraf Gamil Attallah

doi:doi:10.11648/j.bmb.20180305.11

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Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers

El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah

Published in Biochemistry and Molecular Biology (Volume 3, Issue 5)

Received: 10 November 2018 Accepted: 7 December 2018 Published: 10 January 2019

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Abstract

Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.

Published in	Biochemistry and Molecular Biology (Volume 3, Issue 5)
DOI	10.11648/j.bmb.20180305.11
Page(s)	63-70
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Bacillus Thuringiensis, Bacillus Sphaericus, Genetic Diversity, ISSR and SCoT Marker

References

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El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. (2019). Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochemistry and Molecular Biology, 3(5), 63-70. https://doi.org/10.11648/j.bmb.20180305.11

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ACS Style

El-Kawokgy Tahany Mohamed Abou El-Fath; Ibrahim Shafik Darwish; Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem. Mol. Biol. 2019, 3(5), 63-70. doi: 10.11648/j.bmb.20180305.11

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AMA Style

El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem Mol Biol. 2019;3(5):63-70. doi: 10.11648/j.bmb.20180305.11

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@article{10.11648/j.bmb.20180305.11,
  author = {El-Kawokgy Tahany Mohamed Abou El-Fath and Ibrahim Shafik Darwish and Ashraf Gamil Attallah},
  title = {Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {5},
  pages = {63-70},
  doi = {10.11648/j.bmb.20180305.11},
  url = {https://doi.org/10.11648/j.bmb.20180305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180305.11},
  abstract = {Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.},
 year = {2019}
}

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TY  - JOUR
T1  - Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers
AU  - El-Kawokgy Tahany Mohamed Abou El-Fath
AU  - Ibrahim Shafik Darwish
AU  - Ashraf Gamil Attallah
Y1  - 2019/01/10
PY  - 2019
N1  - https://doi.org/10.11648/j.bmb.20180305.11
DO  - 10.11648/j.bmb.20180305.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 63
EP  - 70
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180305.11
AB  - Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.
VL  - 3
IS  - 5
ER  -

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Author Information

El-Kawokgy Tahany Mohamed Abou El-Fath

Microbial Genetics Department, National Research Centre, Giza, Egypt
Ibrahim Shafik Darwish

Molecular Genetics and Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt
Ashraf Gamil Attallah

Microbial Genetics Department, National Research Centre, Giza, Egypt

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El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. (2019). Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochemistry and Molecular Biology, 3(5), 63-70. https://doi.org/10.11648/j.bmb.20180305.11

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El-Kawokgy Tahany Mohamed Abou El-Fath; Ibrahim Shafik Darwish; Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem. Mol. Biol. 2019, 3(5), 63-70. doi: 10.11648/j.bmb.20180305.11

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AMA Style

El-Kawokgy Tahany Mohamed Abou El-Fath, Ibrahim Shafik Darwish, Ashraf Gamil Attallah. Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers. Biochem Mol Biol. 2019;3(5):63-70. doi: 10.11648/j.bmb.20180305.11

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@article{10.11648/j.bmb.20180305.11,
  author = {El-Kawokgy Tahany Mohamed Abou El-Fath and Ibrahim Shafik Darwish and Ashraf Gamil Attallah},
  title = {Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {5},
  pages = {63-70},
  doi = {10.11648/j.bmb.20180305.11},
  url = {https://doi.org/10.11648/j.bmb.20180305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180305.11},
  abstract = {Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.},
 year = {2019}
}

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TY  - JOUR
T1  - Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers
AU  - El-Kawokgy Tahany Mohamed Abou El-Fath
AU  - Ibrahim Shafik Darwish
AU  - Ashraf Gamil Attallah
Y1  - 2019/01/10
PY  - 2019
N1  - https://doi.org/10.11648/j.bmb.20180305.11
DO  - 10.11648/j.bmb.20180305.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 63
EP  - 70
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180305.11
AB  - Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.
VL  - 3
IS  - 5
ER  -

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