Isolation and characterisation of Lactic Acid Bacteria (LAB) associated with the fermentation of maize and sorghum for the production of ogi were evaluated for the development of starter cultures. Changes in pH and LAB counts were investigated during the cereal based-product fermentation. A decrease in pH was observed during the natural fermentation from 0hr to 48hrs. The LAB were isolated, characterised and identified using cultural, morphological and physiological methods. The isolates were: Lactobacillus plantarum, Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei. The isolates from maize–produced ogi were capable of growing on sterilised maize grains, with Lactobacillus plantarum having the highest potential as a starter culture for ogi production from maize. Also, the isolates from sorghum-produced ogi were capable of growing on sterilised sorghum grains, with Lactobacillus delbrueckii subsp. bulgaricus having the higher potential as starter culture for ogi production from sorghum. Sterilised maize grains inoculated with pure culture of Lactobacillus plantarum showed the highest counts of 2.1 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacteria. In the same vein, sterilised sorghum grains inoculated with pure culture of Lactobacillus delbrueckii subsp. bulgaricus showed the higher counts of 1.12 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacterium. There was no significant difference (p ≤ 0.05) in the mean of the pH values obtained during the spontaneous fermentation of maize and sorghum. The results obtained indicated that all the six LAB isolates could be used singly as starter cultures to produce ogi within a shorter fermentation period.
| Published in | Biochemistry and Molecular Biology (Volume 4, Issue 2) |
| DOI | 10.11648/j.bmb.20190402.12 |
| Page(s) | 28-34 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
ogi, Maize, Sorghum, Fermentation, Microbial Evaluation, Lactic Acid Bacteria, Starter Culture
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APA Style
Akin-Osanaiye Bukola Catherine, Kamalu Ikechukwu Okechi. (2019). Evaluation of OGI (CORN CARAMEL) from Maize and Sorghum for Isolation and Characterisation of Lactic Acid Bacteria (LAB). Biochemistry and Molecular Biology, 4(2), 28-34. https://doi.org/10.11648/j.bmb.20190402.12
ACS Style
Akin-Osanaiye Bukola Catherine; Kamalu Ikechukwu Okechi. Evaluation of OGI (CORN CARAMEL) from Maize and Sorghum for Isolation and Characterisation of Lactic Acid Bacteria (LAB). Biochem. Mol. Biol. 2019, 4(2), 28-34. doi: 10.11648/j.bmb.20190402.12
AMA Style
Akin-Osanaiye Bukola Catherine, Kamalu Ikechukwu Okechi. Evaluation of OGI (CORN CARAMEL) from Maize and Sorghum for Isolation and Characterisation of Lactic Acid Bacteria (LAB). Biochem Mol Biol. 2019;4(2):28-34. doi: 10.11648/j.bmb.20190402.12
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Download@article{10.11648/j.bmb.20190402.12,
author = {Akin-Osanaiye Bukola Catherine and Kamalu Ikechukwu Okechi},
title = {Evaluation of OGI (CORN CARAMEL) from Maize and Sorghum for Isolation and Characterisation of Lactic Acid Bacteria (LAB)},
journal = {Biochemistry and Molecular Biology},
volume = {4},
number = {2},
pages = {28-34},
doi = {10.11648/j.bmb.20190402.12},
url = {https://doi.org/10.11648/j.bmb.20190402.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20190402.12},
abstract = {Isolation and characterisation of Lactic Acid Bacteria (LAB) associated with the fermentation of maize and sorghum for the production of ogi were evaluated for the development of starter cultures. Changes in pH and LAB counts were investigated during the cereal based-product fermentation. A decrease in pH was observed during the natural fermentation from 0hr to 48hrs. The LAB were isolated, characterised and identified using cultural, morphological and physiological methods. The isolates were: Lactobacillus plantarum, Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei. The isolates from maize–produced ogi were capable of growing on sterilised maize grains, with Lactobacillus plantarum having the highest potential as a starter culture for ogi production from maize. Also, the isolates from sorghum-produced ogi were capable of growing on sterilised sorghum grains, with Lactobacillus delbrueckii subsp. bulgaricus having the higher potential as starter culture for ogi production from sorghum. Sterilised maize grains inoculated with pure culture of Lactobacillus plantarum showed the highest counts of 2.1 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacteria. In the same vein, sterilised sorghum grains inoculated with pure culture of Lactobacillus delbrueckii subsp. bulgaricus showed the higher counts of 1.12 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacterium. There was no significant difference (p ≤ 0.05) in the mean of the pH values obtained during the spontaneous fermentation of maize and sorghum. The results obtained indicated that all the six LAB isolates could be used singly as starter cultures to produce ogi within a shorter fermentation period.},
year = {2019}
}
TY - JOUR T1 - Evaluation of OGI (CORN CARAMEL) from Maize and Sorghum for Isolation and Characterisation of Lactic Acid Bacteria (LAB) AU - Akin-Osanaiye Bukola Catherine AU - Kamalu Ikechukwu Okechi Y1 - 2019/06/29 PY - 2019 N1 - https://doi.org/10.11648/j.bmb.20190402.12 DO - 10.11648/j.bmb.20190402.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 28 EP - 34 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20190402.12 AB - Isolation and characterisation of Lactic Acid Bacteria (LAB) associated with the fermentation of maize and sorghum for the production of ogi were evaluated for the development of starter cultures. Changes in pH and LAB counts were investigated during the cereal based-product fermentation. A decrease in pH was observed during the natural fermentation from 0hr to 48hrs. The LAB were isolated, characterised and identified using cultural, morphological and physiological methods. The isolates were: Lactobacillus plantarum, Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei. The isolates from maize–produced ogi were capable of growing on sterilised maize grains, with Lactobacillus plantarum having the highest potential as a starter culture for ogi production from maize. Also, the isolates from sorghum-produced ogi were capable of growing on sterilised sorghum grains, with Lactobacillus delbrueckii subsp. bulgaricus having the higher potential as starter culture for ogi production from sorghum. Sterilised maize grains inoculated with pure culture of Lactobacillus plantarum showed the highest counts of 2.1 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacteria. In the same vein, sterilised sorghum grains inoculated with pure culture of Lactobacillus delbrueckii subsp. bulgaricus showed the higher counts of 1.12 × 108 at 24 hrs of fermentation than the other Lactic Acid Bacterium. There was no significant difference (p ≤ 0.05) in the mean of the pH values obtained during the spontaneous fermentation of maize and sorghum. The results obtained indicated that all the six LAB isolates could be used singly as starter cultures to produce ogi within a shorter fermentation period. VL - 4 IS - 2 ER -
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