Experimental Observation on the Retransmission of Radiation Side Effects

Yao Ruoyu; Zhan Hao; Zhang Xuguang

doi:doi:10.11648/j.bmb.20190405.11

| Peer-Reviewed

Experimental Observation on the Retransmission of Radiation Side Effects

Yao Ruoyu, Zhan Hao, Zhang Xuguang

Published in Biochemistry and Molecular Biology (Volume 4, Issue 5)

Received: 8 October 2019 Accepted: 8 November 2019 Published: 15 November 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

[Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p<0.05). After treatment with NUTU19 second-generation medium for 72 h, the apoptosis of NUTU19 and IEC-6 was promoted (p<0.05), and there was no effect on the apoptosis rate of lymphocytes (p>0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p<0.05), and the apoptosis of lymphocytes was decreased (p<0.05). After treated with IEC-6 second-generation medium for 72h, there was no effect on IEC-6 and lymphocyte apoptosis (p>0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.

Published in	Biochemistry and Molecular Biology (Volume 4, Issue 5)
DOI	10.11648/j.bmb.20190405.11
Page(s)	67-73
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Radiation Side Effect, Retransmission, Tumor Cells, Intestinal Epithelial Cells, Lymphocytes

References

[1]	Correspondence Re: H. Nagasawa and J. B. Little, Induction of sister chromatid exchanges by extremely low doses of alpha-particles. Cancer Res., 52: 6394-6396, 1992. Cancer Res 1993; 53: 2188.
[2]	Wersäll PJ, Blomgren H, Pisa P, et al. Regression of non-irradiated metastases after extracranial stereotactic radiotherapy in metastatic renal cell carcinoma. Acta Oncol 2006; 45: 493-497.
[3]	Kulcenty K, Piotrowski I, Zaleska K, et al. Wound fluids collected postoperatively from patients with breast cancer induce epithelial to mesenchymal transition but intraoperative radiotherapy impairs this effect by activating the radiation-induced bystander effect. Sci Rep 2019; 9: 7891.
[4]	Camphausen K, Moses MA, Ménard C, et al. Radiation abscopal antitumor effect is mediated through p53. Cancer Res 2003; 63: 1990-1993.
[5]	Calveley VL, Jelveh S, Langan A, et al. Genistein can mitigate the effect of radiation on rat lung tissue. Radiat Res 2010; 173: 602-611.
[6]	Diot Q, Kavanagh B, Schefter T, et al. Regional normal lung tissue density changes in patients treated with stereotactic body radiation therapy for lung tumors. Int J Radiat Oncol Biol Phys 2012; 84: 1024-1030.
[7]	Chang JY, Zhang X, Wang X, et al. Significant reduction of normal tissue dose by proton radiotherapy compared with three-dimensional conformal or intensity-modulated radiation therapy in Stage I or Stage III non-small-cell lung cancer. Int J Radiat Oncol Biol Phys 2006; 65: 1087-1096.
[8]	Desai S, Kobayashi A, Konishi T, et al. Damaging and protective bystander cross-talk between human lung cancer and normal cells after proton microbeam irradiation. Mutat Res 2014; 763-764: 39-44.
[9]	Siva S, Lobachevsky P, MacManus MP, et al. Radiotherapy for Non-Small Cell Lung Cancer Induces DNA Damage Response in Both Irradiated and Out-of-field Normal Tissues. Clin Cancer Res 2016; 22: 4817-4826.
[10]	Feiock C, Yagi M, Maidman A, et al. Central Nervous System Injury - A Newly Observed Bystander Effect of Radiation. PLoS One 2016; 11: e0163233.
[11]	Dong C, He M, Tu W, et al. The differential role of human macrophage in triggering secondary bystander effects after either gamma-ray or carbon beam irradiation. Cancer Lett 2015; 363: 92-100.
[12]	Fu J, Yuan D, Xiao L, et al. The crosstalk between α-irradiated Beas-2B cells and its bystander U937 cells through MAPK and NF-κB signaling pathways. Mutat Res 2016; 783: 1-8.
[13]	Yang S, Xu J, Shao W, et al. Radiation-Induced Bystander Effects in A549 Cells Exposed to 6 MV X-rays. Cell Biochem Biophys 2015; 72: 877-882.
[14]	Kong EY, Cheng SH. Induction of autophagy and interleukin 6 secretion in bystander cells: metabolic cooperation for radiation-induced rescue effect?J Radiat Res 2018; 59: 129-140.
[15]	Bill MA, Srivastava K, Breen C, et al. Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells. Oncotarget 2017; 8: 97331-97343.
[16]	Khan MA, Hill RP. Partial volume rat lung irradiation: an evaluation of early DNA damage. Int J Radiat Oncol Biol Phys 1998; 40: 467-476.
[17]	Koturbash I, Rugo RE, Hendricks CA, et al. Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo. Oncogene 2006; 25: 4267-4275.
[18]	Koturbash I, Zemp FJ, Kutanzi K, et al. Sex-specific microRNAome deregulation in the shielded bystander spleen of cranially exposed mice. Cell Cycle 2008; 7: 1658-1667.
[19]	Tamminga J, Koturbash I, Baker M, et al. Paternal cranial irradiation induces distant bystander DNA damage in the germline and leads to epigenetic alterations in the offspring. Cell Cycle 2008; 7: 1238-1245.
[20]	Ilnytskyy Y, Koturbash I. Radiation-induced bystander effects in vivo are epigenetically regulated in a tissue-specific manner. Environ Mol Mutagen 2009; 50: 105-113.
[21]	Sun DQ, Xiao Y, Han L, et al. High expression of AP1S1 in radiation side effect and its role in side effects. Radioprotection 2010, 30 (6): 356.
[22]	Herrera FG, Bourhis J. Radiotherapy combination opportunities leveraging immunity for the next oncology practice. CA Cancer J Clin 2017; 67: 65-85.
[23]	Tang D, Kang R, Zeh HJ. High-mobility group box 1, oxidative stress, and disease. Antioxid Redox Signal 2011; 14: 1315-1335.
[24]	Garg AD, Krysko DV, Verfaillie T, et al. A novel pathway combining calreticulin exposure and ATP secretion in immunogenic cancer cell death. EMBO J 2012; 31: 1062-1079.
[25]	Ohshima Y, Tsukimoto M, Takenouchi T, et al. gamma-Irradiation induces P2X (7) receptor-dependent ATP release from B16 melanoma cells. Biochim Biophys Acta 2010; 1800: 40-46.
[26]	Elliott MR, Chekeni FB, Trampont PC, et al. Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance. Nature 2009; 461: 282-286.
[27]	Mukherjee S. Radiation-induced bystander phenomenon: insight and implications in radiotherapy. Int J Radiat Biol 2019; 95: 243-263.
[28]	Zhang X, Pan Y, Shao CL. Research progress on the bystander effect of radiotherapy in vivo. International Journal of Radiation Medicine and Nuclear Medicine 2017 (41) 3: 209-213.

Cite This Article

Plain Text BibTeX RIS

APA Style

Yao Ruoyu, Zhan Hao, Zhang Xuguang. (2019). Experimental Observation on the Retransmission of Radiation Side Effects. Biochemistry and Molecular Biology, 4(5), 67-73. https://doi.org/10.11648/j.bmb.20190405.11

Copy | Download

ACS Style

Yao Ruoyu; Zhan Hao; Zhang Xuguang. Experimental Observation on the Retransmission of Radiation Side Effects. Biochem. Mol. Biol. 2019, 4(5), 67-73. doi: 10.11648/j.bmb.20190405.11

Copy | Download

AMA Style

Yao Ruoyu, Zhan Hao, Zhang Xuguang. Experimental Observation on the Retransmission of Radiation Side Effects. Biochem Mol Biol. 2019;4(5):67-73. doi: 10.11648/j.bmb.20190405.11

Copy | Download

@article{10.11648/j.bmb.20190405.11,
  author = {Yao Ruoyu and Zhan Hao and Zhang Xuguang},
  title = {Experimental Observation on the Retransmission of Radiation Side Effects},
  journal = {Biochemistry and Molecular Biology},
  volume = {4},
  number = {5},
  pages = {67-73},
  doi = {10.11648/j.bmb.20190405.11},
  url = {https://doi.org/10.11648/j.bmb.20190405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20190405.11},
  abstract = {[Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Experimental Observation on the Retransmission of Radiation Side Effects
AU  - Yao Ruoyu
AU  - Zhan Hao
AU  - Zhang Xuguang
Y1  - 2019/11/15
PY  - 2019
N1  - https://doi.org/10.11648/j.bmb.20190405.11
DO  - 10.11648/j.bmb.20190405.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 67
EP  - 73
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20190405.11
AB  - [Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.
VL  - 4
IS  - 5
ER  -

Copy | Download

Author Information

Yao Ruoyu

Graduate School of Xuzhou Medical University, Xuzhou, China
Zhan Hao

Xuzhou Tumor Hospital, Xuzhou, China
Zhang Xuguang

Graduate School of Xuzhou Medical University, Xuzhou, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Yao Ruoyu, Zhan Hao, Zhang Xuguang. (2019). Experimental Observation on the Retransmission of Radiation Side Effects. Biochemistry and Molecular Biology, 4(5), 67-73. https://doi.org/10.11648/j.bmb.20190405.11

Copy | Download

ACS Style

Yao Ruoyu; Zhan Hao; Zhang Xuguang. Experimental Observation on the Retransmission of Radiation Side Effects. Biochem. Mol. Biol. 2019, 4(5), 67-73. doi: 10.11648/j.bmb.20190405.11

Copy | Download

AMA Style

Yao Ruoyu, Zhan Hao, Zhang Xuguang. Experimental Observation on the Retransmission of Radiation Side Effects. Biochem Mol Biol. 2019;4(5):67-73. doi: 10.11648/j.bmb.20190405.11

Copy | Download

@article{10.11648/j.bmb.20190405.11,
  author = {Yao Ruoyu and Zhan Hao and Zhang Xuguang},
  title = {Experimental Observation on the Retransmission of Radiation Side Effects},
  journal = {Biochemistry and Molecular Biology},
  volume = {4},
  number = {5},
  pages = {67-73},
  doi = {10.11648/j.bmb.20190405.11},
  url = {https://doi.org/10.11648/j.bmb.20190405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20190405.11},
  abstract = {[Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Experimental Observation on the Retransmission of Radiation Side Effects
AU  - Yao Ruoyu
AU  - Zhan Hao
AU  - Zhang Xuguang
Y1  - 2019/11/15
PY  - 2019
N1  - https://doi.org/10.11648/j.bmb.20190405.11
DO  - 10.11648/j.bmb.20190405.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 67
EP  - 73
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20190405.11
AB  - [Objective] To observe the retransmission of radiation side effects between cells. [Materials and Methods] The mouse ovarian cancer cell line NUTU19 was irradiated with 6MV-X-rays, and the culture medium was prepared for the first-generation conditioned medium. The first-generation effector cells were used to detect the NUTU19 cell line and the intestinal mucosal epithelial IEC-6 cell line. The secretion of effector cells was a second-generation conditioned medium, and the second-generation effector cells NUTU19, IEC-6, and mouse lymphocytes were treated to measure cell viability and apoptosis. [Results] After treated with NUTU19 second-generation medium for 48 hours, the apoptosis rate of IEC-6 and NUTU19 cells was promoted (p>0.05), and the apoptosis rate of lymphocytes was decreased (p0.05). After treatment with IEC-6 second-generation medium for 48h, the apoptosis of NUTU19 and IEC-6 was promoted (p0.05). It promoted the apoptosis of NUTU19 (p<0.05). [Conclusion]: Under certain conditions, tumor cells, intestinal epithelial cells and lymphocytes can retransmit the damage of the side effect of radiation.
VL  - 4
IS  - 5
ER  -

Copy | Download