The purpose of this study was to investigate the expression of interleukins (ILs) cytokine in postauricular skin, mastoid mucosa, inflamed mastoid mucosa and middle ear mucosa from Otitis patients. Specimens were obtained from patients with otitis media who visited the Department of Otorhinolaryngology, Chungju Hospital, Kunkuk University School of Medicine, Chungju, Republic of Korea between August 2015 and July 2017. We examined human 38 kinds of human interleukins in this experiment. The total RNA was extracted from specimens using RNAiso Plus (Takara, Japan) according to the manufacturer's instructions. 38 ILs primers were used. IL-4, IL-8, IL-9, IL-21, IL-24 were not expressed at postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were strongly expressed in mastoid mucosa and middle ear mucosa. The degree of IL-7 gene expression may be an indicator gene in otitis media. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were involved in the inflammatory and immune processes of otitis media.
| Published in | Biochemistry and Molecular Biology (Volume 4, Issue 4) |
| DOI | 10.11648/j.bmb.20190404.12 |
| Page(s) | 59-66 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Otitis Media, Interleukins (ILs), Inflamed Mastoid Mucosa, Middle Ear Mucosa
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APA Style
Sung Ho Kang, Dae Joon Lim, Bo Hyung Kim, Myung Sang Yu, Shinseok Kang. (2019). Expression of Interleukins (ILs) mRNA in Otitis Media. Biochemistry and Molecular Biology, 4(4), 59-66. https://doi.org/10.11648/j.bmb.20190404.12
ACS Style
Sung Ho Kang; Dae Joon Lim; Bo Hyung Kim; Myung Sang Yu; Shinseok Kang. Expression of Interleukins (ILs) mRNA in Otitis Media. Biochem. Mol. Biol. 2019, 4(4), 59-66. doi: 10.11648/j.bmb.20190404.12
AMA Style
Sung Ho Kang, Dae Joon Lim, Bo Hyung Kim, Myung Sang Yu, Shinseok Kang. Expression of Interleukins (ILs) mRNA in Otitis Media. Biochem Mol Biol. 2019;4(4):59-66. doi: 10.11648/j.bmb.20190404.12
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Download@article{10.11648/j.bmb.20190404.12,
author = {Sung Ho Kang and Dae Joon Lim and Bo Hyung Kim and Myung Sang Yu and Shinseok Kang},
title = {Expression of Interleukins (ILs) mRNA in Otitis Media},
journal = {Biochemistry and Molecular Biology},
volume = {4},
number = {4},
pages = {59-66},
doi = {10.11648/j.bmb.20190404.12},
url = {https://doi.org/10.11648/j.bmb.20190404.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20190404.12},
abstract = {The purpose of this study was to investigate the expression of interleukins (ILs) cytokine in postauricular skin, mastoid mucosa, inflamed mastoid mucosa and middle ear mucosa from Otitis patients. Specimens were obtained from patients with otitis media who visited the Department of Otorhinolaryngology, Chungju Hospital, Kunkuk University School of Medicine, Chungju, Republic of Korea between August 2015 and July 2017. We examined human 38 kinds of human interleukins in this experiment. The total RNA was extracted from specimens using RNAiso Plus (Takara, Japan) according to the manufacturer's instructions. 38 ILs primers were used. IL-4, IL-8, IL-9, IL-21, IL-24 were not expressed at postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were strongly expressed in mastoid mucosa and middle ear mucosa. The degree of IL-7 gene expression may be an indicator gene in otitis media. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were involved in the inflammatory and immune processes of otitis media.},
year = {2019}
}
TY - JOUR T1 - Expression of Interleukins (ILs) mRNA in Otitis Media AU - Sung Ho Kang AU - Dae Joon Lim AU - Bo Hyung Kim AU - Myung Sang Yu AU - Shinseok Kang Y1 - 2019/10/25 PY - 2019 N1 - https://doi.org/10.11648/j.bmb.20190404.12 DO - 10.11648/j.bmb.20190404.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 59 EP - 66 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20190404.12 AB - The purpose of this study was to investigate the expression of interleukins (ILs) cytokine in postauricular skin, mastoid mucosa, inflamed mastoid mucosa and middle ear mucosa from Otitis patients. Specimens were obtained from patients with otitis media who visited the Department of Otorhinolaryngology, Chungju Hospital, Kunkuk University School of Medicine, Chungju, Republic of Korea between August 2015 and July 2017. We examined human 38 kinds of human interleukins in this experiment. The total RNA was extracted from specimens using RNAiso Plus (Takara, Japan) according to the manufacturer's instructions. 38 ILs primers were used. IL-4, IL-8, IL-9, IL-21, IL-24 were not expressed at postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were strongly expressed in mastoid mucosa and middle ear mucosa. The degree of IL-7 gene expression may be an indicator gene in otitis media. IL-1a, IL-17, IL-17d, IL-17e and IL-17f were involved in the inflammatory and immune processes of otitis media. VL - 4 IS - 4 ER -
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