Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum <i>in vitro</i>

Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Mari Kimoto; Kazuo Toda; Masato Ota

doi:doi:10.11648/j.ijnfs.20180703.13

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Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, Kazuo Toda, Masato Ota

Published in International Journal of Nutrition and Food Sciences (Volume 7, Issue 3)

Received: 7 April 2018 Accepted: 27 April 2018 Published: 18 May 2018

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Abstract

Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.

Published in	International Journal of Nutrition and Food Sciences (Volume 7, Issue 3)
DOI	10.11648/j.ijnfs.20180703.13
Page(s)	94-99
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Thiamine, Quinine, Movement, Small Intestine, Mouse

References

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Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. (2018). Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. International Journal of Nutrition and Food Sciences, 7(3), 94-99. https://doi.org/10.11648/j.ijnfs.20180703.13

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ACS Style

Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int. J. Nutr. Food Sci. 2018, 7(3), 94-99. doi: 10.11648/j.ijnfs.20180703.13

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AMA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int J Nutr Food Sci. 2018;7(3):94-99. doi: 10.11648/j.ijnfs.20180703.13

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@article{10.11648/j.ijnfs.20180703.13,
  author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Mari Kimoto and Kazuo Toda and Masato Ota},
  title = {Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {7},
  number = {3},
  pages = {94-99},
  doi = {10.11648/j.ijnfs.20180703.13},
  url = {https://doi.org/10.11648/j.ijnfs.20180703.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20180703.13},
  abstract = {Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.},
 year = {2018}
}

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TY  - JOUR
T1  - Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro
AU  - Atsuko Yamashita
AU  - Nana Shimamoto
AU  - Kyoko Morita
AU  - Hasumi Sugiyama
AU  - Mari Kimoto
AU  - Kazuo Toda
AU  - Masato Ota
Y1  - 2018/05/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ijnfs.20180703.13
DO  - 10.11648/j.ijnfs.20180703.13
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 94
EP  - 99
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20180703.13
AB  - Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.
VL  - 7
IS  - 3
ER  -

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Author Information

Atsuko Yamashita

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan
Nana Shimamoto

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan
Kyoko Morita

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan
Hasumi Sugiyama

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan
Mari Kimoto

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan
Kazuo Toda

Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
Masato Ota

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

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APA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. (2018). Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. International Journal of Nutrition and Food Sciences, 7(3), 94-99. https://doi.org/10.11648/j.ijnfs.20180703.13

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ACS Style

Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int. J. Nutr. Food Sci. 2018, 7(3), 94-99. doi: 10.11648/j.ijnfs.20180703.13

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AMA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int J Nutr Food Sci. 2018;7(3):94-99. doi: 10.11648/j.ijnfs.20180703.13

Copy | Download

@article{10.11648/j.ijnfs.20180703.13,
  author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Mari Kimoto and Kazuo Toda and Masato Ota},
  title = {Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {7},
  number = {3},
  pages = {94-99},
  doi = {10.11648/j.ijnfs.20180703.13},
  url = {https://doi.org/10.11648/j.ijnfs.20180703.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20180703.13},
  abstract = {Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro
AU  - Atsuko Yamashita
AU  - Nana Shimamoto
AU  - Kyoko Morita
AU  - Hasumi Sugiyama
AU  - Mari Kimoto
AU  - Kazuo Toda
AU  - Masato Ota
Y1  - 2018/05/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ijnfs.20180703.13
DO  - 10.11648/j.ijnfs.20180703.13
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 94
EP  - 99
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20180703.13
AB  - Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.
VL  - 7
IS  - 3
ER  -

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