Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations

Idrees F. Al-Momani; Majdoleen H. Rababah

doi:doi:10.11648/j.ijpc.20170304.11

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Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations

Idrees F. Al-Momani, Majdoleen H. Rababah

Published in International Journal of Pharmacy and Chemistry (Volume 3, Issue 4)

Received: 1 June 2017 Accepted: 16 June 2017 Published: 21 July 2017

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Abstract

A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.

Published in	International Journal of Pharmacy and Chemistry (Volume 3, Issue 4)
DOI	10.11648/j.ijpc.20170304.11
Page(s)	52-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Omeprazole, Flow Injection Analysis, Pharmaceutical Products, Human Plasma

References

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[2]	Burham T. H. (2001). Drug Facts and Comparisons Hebel, S. K., St. Louis. A Wolters Kluwer.
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[4]	Macek J., Ptacek P., Klima J. (1997). Determination of omeprazole in human plasma by high performance liquid chromatography. J. of Chromatography B, 689:239-243.
[5]	Wang J., Wang Y., Fawcett J. P., Wang Y., Gu J. (2005). Determination of omeprazole in human plasma by liquid chromatography-electrospray quadrupole linear ion trap mass spectrometry. J Pharm Biomed Anal. 2005 Sep 15; 39(3-4):631-5.
[6]	Shahrokhian S., Ghalkhani M., Bayat M., Ghorbani-Bidkorbeh F. (2015). Voltammetric behavior and determination of trace amounts of omeprazole using an edge-plane Pyrolytic graphite electrode. Iranian Journal of Pharmaceutical Research (2015), 14 (2): 465-471.
[7]	Al-Momani I. F., Rababah M. H. (2010). Validation of HPLC and FIA spectrophotometric methods for the determination of lansoprazole in pharmaceutical dosage forms and human plasma. American Journal of Analytical Chemistry, 2010, 1, 34-39.
[8]	Kumaraswamy D., Stephenrathinaraj B., Rajveer C. H., Sudharshini S., Shrestha B., Rao R. (2010). Process validation of analytical method development and validation for omeprazole capsules and blend. International Journal of Pharma and Bio Sciences, 1(2), 1-6.
[9]	Al-Momani I. F. (2001). Spectrophotometric determination of selected cephalosporins in drug formulations using flow injection analysis Journal of Pharmaceutical and Biomedical Analysis, 25, 751–757.
[10]	Klyankar T., Wadher S. J., Gholve R., Anitha K. (2016). UV Spectrophotometric estimation of diclofenac potassium and omeprazole magnesium in bulk and combined tablet dosage form. International Journal of MediPharm Research, 2(1), 42-53.
[11]	Syed A. A., Syeda A. (2008). Spectrophotometric determination of certain benzimidazole proton pump inhibitors, Indian J. Pharm. Sci., 2008, 70 (4): 507-510.
[12]	Al-Momani I. F. (2004). Flow-Injection spectrophotometric determination of amoxcillin, cephalexin, ampicillin, and cephradine in pharmaceutical formulations. Analytical Letters, 37(10), 2099–2110.
[13]	Al-Momani I. F. (2006). Flow Injection spectrophotometric determination of the antibacterial levofloxacin in tablets and human urine. Analytical Letters, 39: 741–750.
[14]	Al-Momani I. F. (2006). Indirect Flow-Injection spectrophotometric determination of meloxicam, tenoxicam and piroxicam in pharmaceutical formulations. Analytical Sciences, 22, 1611-1614.
[15]	Al-Momani I. F., Kanan S. J. (2008). Flow-Injection spectrophotometric and LC determination of doxycycline, oxytetracycline and chlortetracycline in biological fluids and pharmaceutical preparations. Flow Injection Analysis 25(1), 29–34.
[16]	Al-Momani I. F., Haj-Hussein A. T., Tahtamouni A. N., J.) 2008). Flow injection spectrophotometric and chromatographic determination of ciprofloxacin and norfloxacin in pharmaceutical formulations. Flow Injection Analysis 25(2), 151–155.
[17]	Haj-Hussein A. T., Al-Momani I. F. (1989). Indirect spectrophotometric determination of fluoride in water with zirconium-SPADNS by flow injection analysis, Analytical Letters 22(6), 1581-1599.
[18]	Al-Momani I. F., Awni I., Khalil H. S., Esmadi F. (1999). Spectrophotometric determination of cefadroxil in drug formulations using flow injection analysis, Analytical Letters 32(15), 2988-2977.
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APA Style

Idrees F. Al-Momani, Majdoleen H. Rababah. (2017). Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. International Journal of Pharmacy and Chemistry, 3(4), 52-55. https://doi.org/10.11648/j.ijpc.20170304.11

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ACS Style

Idrees F. Al-Momani; Majdoleen H. Rababah. Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. Int. J. Pharm. Chem. 2017, 3(4), 52-55. doi: 10.11648/j.ijpc.20170304.11

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AMA Style

Idrees F. Al-Momani, Majdoleen H. Rababah. Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. Int J Pharm Chem. 2017;3(4):52-55. doi: 10.11648/j.ijpc.20170304.11

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@article{10.11648/j.ijpc.20170304.11,
  author = {Idrees F. Al-Momani and Majdoleen H. Rababah},
  title = {Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {3},
  number = {4},
  pages = {52-55},
  doi = {10.11648/j.ijpc.20170304.11},
  url = {https://doi.org/10.11648/j.ijpc.20170304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20170304.11},
  abstract = {A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.},
 year = {2017}
}

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TY  - JOUR
T1  - Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations
AU  - Idrees F. Al-Momani
AU  - Majdoleen H. Rababah
Y1  - 2017/07/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijpc.20170304.11
DO  - 10.11648/j.ijpc.20170304.11
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 52
EP  - 55
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20170304.11
AB  - A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.
VL  - 3
IS  - 4
ER  -

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Author Information

Idrees F. Al-Momani

Chemistry Department, Yarmouk University, Irbid, Jordan
Majdoleen H. Rababah

Chemistry Department, Yarmouk University, Irbid, Jordan

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APA Style

Idrees F. Al-Momani, Majdoleen H. Rababah. (2017). Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. International Journal of Pharmacy and Chemistry, 3(4), 52-55. https://doi.org/10.11648/j.ijpc.20170304.11

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ACS Style

Idrees F. Al-Momani; Majdoleen H. Rababah. Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. Int. J. Pharm. Chem. 2017, 3(4), 52-55. doi: 10.11648/j.ijpc.20170304.11

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AMA Style

Idrees F. Al-Momani, Majdoleen H. Rababah. Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations. Int J Pharm Chem. 2017;3(4):52-55. doi: 10.11648/j.ijpc.20170304.11

Copy | Download

@article{10.11648/j.ijpc.20170304.11,
  author = {Idrees F. Al-Momani and Majdoleen H. Rababah},
  title = {Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {3},
  number = {4},
  pages = {52-55},
  doi = {10.11648/j.ijpc.20170304.11},
  url = {https://doi.org/10.11648/j.ijpc.20170304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20170304.11},
  abstract = {A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Automated Flow Injection Spectrophotometric Determination of the Proton Pump Inhibitor Omeprazole in Pharmaceutical Formulations
AU  - Idrees F. Al-Momani
AU  - Majdoleen H. Rababah
Y1  - 2017/07/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijpc.20170304.11
DO  - 10.11648/j.ijpc.20170304.11
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 52
EP  - 55
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20170304.11
AB  - A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.
VL  - 3
IS  - 4
ER  -

Copy | Download