Abstract: A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active material was detected at 370 nm. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 2.0 and 6.5 μg/ml, respectively. No interference was observed from common pharmaceutical additives. The method was successfully applied to the assay of omeprazole in capsule preparations and in spiked human plasma. The results were statistically compared with those of the official high-performance liquid chromatography (HPLC) method of the USP by applying Student’s t-test and F-test.Abstract: A simple and fast flow-injection method for the determination of omeprazole in pharmaceutical preparations is proposed. Detection of the drug is based on the measurement of the absorption signals for the product resulting from the oxidation of omeprazole with N-bromosuccinimide in acidic medium. A flow rate of 0.5 ml/min was pumped and active mater...Show More
Abstract: Present study was carried out to investigate in vitro anti-bacterial and anti-fungal activity from the seeds of an Indian traditional medicinal plant Cassia tora. Plant material was separated and successively extracted with various organic solvents. Extracts was evaluated for solubility, moisture content, melting point, FTIR and other qualitative analysis for Photo constituents. In vitro antibacterial and antifungal studies were carried out by disc diffusion method. Two test samples were prepared in concentrations of 100mg/ml. Extract was found efficacious against various strains of bacteria and fungal species. 100mg/ml test sample shows better zone of inhibitions in bacterial strains. It was obtained in the range of 16.67 to 23.00 among them maximum inhibition was observed in pseudomonasaeruginosa (gram positive bacteria) and last inhibition was observed in E. coli (gram negative). It has also shown satisfactory inhibition in fungal species like Candida albicans and Candida glabrata. Thus, cassia tora can be considered as a safer and efficacious herbal candidate for antimicrobial formulation.Abstract: Present study was carried out to investigate in vitro anti-bacterial and anti-fungal activity from the seeds of an Indian traditional medicinal plant Cassia tora. Plant material was separated and successively extracted with various organic solvents. Extracts was evaluated for solubility, moisture content, melting point, FTIR and other qualitative a...Show More