Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect.
| Published in | International Journal of Pharmacy and Chemistry (Volume 8, Issue 1) |
| DOI | 10.11648/j.ijpc.20220801.12 |
| Page(s) | 5-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Traditional Mongolian Medicine Eerdun Wurile, Molecular Modeling Technology, Genipin, MAPK, PC12 Cells
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APA Style
Wang Qingliang, Bao Yinping, Sudun, Gaowa, Dugeer, et al. (2022). The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. International Journal of Pharmacy and Chemistry, 8(1), 5-11. https://doi.org/10.11648/j.ijpc.20220801.12
ACS Style
Wang Qingliang; Bao Yinping; Sudun; Gaowa; Dugeer, et al. The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. Int. J. Pharm. Chem. 2022, 8(1), 5-11. doi: 10.11648/j.ijpc.20220801.12
AMA Style
Wang Qingliang, Bao Yinping, Sudun, Gaowa, Dugeer, et al. The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells. Int J Pharm Chem. 2022;8(1):5-11. doi: 10.11648/j.ijpc.20220801.12
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Download@article{10.11648/j.ijpc.20220801.12,
author = {Wang Qingliang and Bao Yinping and Sudun and Gaowa and Dugeer and Alatengqimuge and Li Hanqing and Song Fushun and Zhang Wen and Tianji and Bao Xilinqiqige and Tegexibaiyin and Bao Siqin and Wuhanqimuge},
title = {The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells},
journal = {International Journal of Pharmacy and Chemistry},
volume = {8},
number = {1},
pages = {5-11},
doi = {10.11648/j.ijpc.20220801.12},
url = {https://doi.org/10.11648/j.ijpc.20220801.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220801.12},
abstract = {Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect.},
year = {2022}
}
TY - JOUR T1 - The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells AU - Wang Qingliang AU - Bao Yinping AU - Sudun AU - Gaowa AU - Dugeer AU - Alatengqimuge AU - Li Hanqing AU - Song Fushun AU - Zhang Wen AU - Tianji AU - Bao Xilinqiqige AU - Tegexibaiyin AU - Bao Siqin AU - Wuhanqimuge Y1 - 2022/01/28 PY - 2022 N1 - https://doi.org/10.11648/j.ijpc.20220801.12 DO - 10.11648/j.ijpc.20220801.12 T2 - International Journal of Pharmacy and Chemistry JF - International Journal of Pharmacy and Chemistry JO - International Journal of Pharmacy and Chemistry SP - 5 EP - 11 PB - Science Publishing Group SN - 2575-5749 UR - https://doi.org/10.11648/j.ijpc.20220801.12 AB - Background: Traditional Mongolian medicine Eerdun Wurile is one of the ideal drugs for cerebral stroke and is effective on treating vascular and nervous system diseases. Numerous studies have proved that Eerdun Wurile has neuroprotective effect. However, the biological mechanism of which is still unclear. Objective: To investigatethe molecular mechanism underlying the neuroprotective effect of Eerdun Wurile. Methods: The extract from Eerdun Wurile was prepared by simulated gastric fluid first and its effect on PC12 cells differentiaton and MAPK phosphorylation was tested by neurite outgrowth assay and western blotting. Also, the target of active compounds was predicted by moleculr modeling system and the content of active compounds was examined by LC-MS/MS. Result: Result showed that the extract stimulated the differentiation of PC12 cells, exhibited nerve growth factor (NGF)-like effect. Meanwhile, the extract induced the MAPK phosphorylation in PC12 cells. In-depth analysis by molecular modeling technology predicted that genipin interact with MAPK as a target. Consistent with the prediction, genipin induced the phosphorylation of MAPK in PC12 cells. Besides, genipin was detected both in the extract and Eerdun Wurile pills. Conclusion: These results proved that the extract of Eerdun Wurile has neuroprotective effect in PC12 cells, and genipin in the extract and Eerdun Wurile might be one of the active compounds that show neuroprotective effect. VL - 8 IS - 1 ER -
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