Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase

Hameed Akbar; Divine Mensah Sedzro; Mazhar Khan; Sm Faysal Bellah; S M Saker Billah

doi:doi:10.11648/j.jcebe.20180202.13

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Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase

Hameed Akbar, Divine Mensah Sedzro, Mazhar Khan, Sm Faysal Bellah, S M Saker Billah

Published in Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 2)

Received: 14 October 2018 Accepted: 29 October 2018 Published: 27 November 2018

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Abstract

An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today’s world. This enzyme can catalyze peroxide based reactions. Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. These enzymes can also oxidatively breakdown the synthetic azo dyes. One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. On the other hand, if co-immobilized with H₂O₂–producing enzyme, it can be used for determination of range of compounds e.g. alcohols, choline, glucose and glutamate. Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits.

Published in	Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 2)
DOI	10.11648/j.jcebe.20180202.13
Page(s)	52-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Horseradish Peroxidase, Biochemical Assays, Bioremediation, Bio-Bleaching, Bio-Pulping

References

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APA Style

Hameed Akbar, Divine Mensah Sedzro, Mazhar Khan, Sm Faysal Bellah, S M Saker Billah. (2018). Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. Journal of Chemical, Environmental and Biological Engineering, 2(2), 52-59. https://doi.org/10.11648/j.jcebe.20180202.13

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ACS Style

Hameed Akbar; Divine Mensah Sedzro; Mazhar Khan; Sm Faysal Bellah; S M Saker Billah. Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. J. Chem. Environ. Biol. Eng. 2018, 2(2), 52-59. doi: 10.11648/j.jcebe.20180202.13

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AMA Style

Hameed Akbar, Divine Mensah Sedzro, Mazhar Khan, Sm Faysal Bellah, S M Saker Billah. Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. J Chem Environ Biol Eng. 2018;2(2):52-59. doi: 10.11648/j.jcebe.20180202.13

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@article{10.11648/j.jcebe.20180202.13,
  author = {Hameed Akbar and Divine Mensah Sedzro and Mazhar Khan and Sm Faysal Bellah and S M Saker Billah},
  title = {Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {2},
  pages = {52-59},
  doi = {10.11648/j.jcebe.20180202.13},
  url = {https://doi.org/10.11648/j.jcebe.20180202.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180202.13},
  abstract = {An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today’s world. This enzyme can catalyze peroxide based reactions. Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. These enzymes can also oxidatively breakdown the synthetic azo dyes. One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. On the other hand, if co-immobilized with H2O2–producing enzyme, it can be used for determination of range of compounds e.g. alcohols, choline, glucose and glutamate. Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits.},
 year = {2018}
}

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TY  - JOUR
T1  - Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase
AU  - Hameed Akbar
AU  - Divine Mensah Sedzro
AU  - Mazhar Khan
AU  - Sm Faysal Bellah
AU  - S M Saker Billah
Y1  - 2018/11/27
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180202.13
DO  - 10.11648/j.jcebe.20180202.13
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 52
EP  - 59
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180202.13
AB  - An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today’s world. This enzyme can catalyze peroxide based reactions. Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. These enzymes can also oxidatively breakdown the synthetic azo dyes. One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. On the other hand, if co-immobilized with H2O2–producing enzyme, it can be used for determination of range of compounds e.g. alcohols, choline, glucose and glutamate. Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits.
VL  - 2
IS  - 2
ER  -

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Author Information

Hameed Akbar

Laboratory of Cellular Dynamics, School of Life Science, University of Science and Technology of China, Hefei, China
Divine Mensah Sedzro

Laboratory of Cellular Dynamics, School of Life Science, University of Science and Technology of China, Hefei, China
Mazhar Khan

Molecular & Cytogenetics Lab, School of Life Science, University of Science and Technology of China, Hefei, P R China
Sm Faysal Bellah

Laboratory of Cellular Dynamics, School of Life Science, University of Science and Technology of China, Hefei, China
S M Saker Billah

Department of Chemistry, Govt. M. M. University College, Jessore, Bangladesh

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APA Style

Hameed Akbar, Divine Mensah Sedzro, Mazhar Khan, Sm Faysal Bellah, S M Saker Billah. (2018). Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. Journal of Chemical, Environmental and Biological Engineering, 2(2), 52-59. https://doi.org/10.11648/j.jcebe.20180202.13

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ACS Style

Hameed Akbar; Divine Mensah Sedzro; Mazhar Khan; Sm Faysal Bellah; S M Saker Billah. Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. J. Chem. Environ. Biol. Eng. 2018, 2(2), 52-59. doi: 10.11648/j.jcebe.20180202.13

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AMA Style

Hameed Akbar, Divine Mensah Sedzro, Mazhar Khan, Sm Faysal Bellah, S M Saker Billah. Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase. J Chem Environ Biol Eng. 2018;2(2):52-59. doi: 10.11648/j.jcebe.20180202.13

Copy | Download

@article{10.11648/j.jcebe.20180202.13,
  author = {Hameed Akbar and Divine Mensah Sedzro and Mazhar Khan and Sm Faysal Bellah and S M Saker Billah},
  title = {Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {2},
  pages = {52-59},
  doi = {10.11648/j.jcebe.20180202.13},
  url = {https://doi.org/10.11648/j.jcebe.20180202.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180202.13},
  abstract = {An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today’s world. This enzyme can catalyze peroxide based reactions. Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. These enzymes can also oxidatively breakdown the synthetic azo dyes. One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. On the other hand, if co-immobilized with H2O2–producing enzyme, it can be used for determination of range of compounds e.g. alcohols, choline, glucose and glutamate. Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Structure, Function and Applications of a Classic Enzyme: Horseradish Peroxidase
AU  - Hameed Akbar
AU  - Divine Mensah Sedzro
AU  - Mazhar Khan
AU  - Sm Faysal Bellah
AU  - S M Saker Billah
Y1  - 2018/11/27
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180202.13
DO  - 10.11648/j.jcebe.20180202.13
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 52
EP  - 59
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180202.13
AB  - An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today’s world. This enzyme can catalyze peroxide based reactions. Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. These enzymes can also oxidatively breakdown the synthetic azo dyes. One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. On the other hand, if co-immobilized with H2O2–producing enzyme, it can be used for determination of range of compounds e.g. alcohols, choline, glucose and glutamate. Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits.
VL  - 2
IS  - 2
ER  -

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