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Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine

Received: 31 December 2016     Accepted: 12 January 2017     Published: 6 February 2017
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Abstract

A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.

Published in Science Journal of Analytical Chemistry (Volume 5, Issue 1)
DOI 10.11648/j.sjac.20170501.11
Page(s) 1-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Gemifloxacin Mesylate, Spectrofluorometry, Terbium(III), Hexamine, Ternary Complex

References
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[3] B. A. Moussa, M. A. Mahrouse, M. A. Hassan and M. G. Fawzy. (2013). Stability indicating spectrophotometric and TLC densitometric methods for the determination of gemifloxacin mesylate in tablet form, Anal. Chem. Indian J. 12: 165–176.
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[5] R. El-Bagary, N. F. Abo-Talib and M. B. N. Eldin. (2011). Validated stability indicating assay of gemifloxacin by different chromatographic and spectrophotometric methods of analysis, J. Chem. Pharm. Res. 3: 562–570.
[6] S. S. Panda, B. V. V. Ravi Kumar, K. S. Rao, V. R. Kumar and D. Patanaik. (2011). Difference spectrophotometric determination of gemifloxacin mesylate in tablet formulation, Asian J. Biochem. Pharm. Res. 1: 442–447.
[7] K. B. C. Sekhar, D. Madhuri and N. Devanna. (2010). Direct and derivative spectrophotometric determination of gemifloxacin mesylate via metal chelate, Acta Cienc. Indica, Ser. Chem. 36: 165– 171.
[8] R. Rote Ambadas and S. P. Pingle. (2010). Validated UV-spectrophotometric methods for determination of gemifloxacin mesylate in pharmaceutical tablet dosage forms, E-J. Chem. 7: S344–S348.
[9] D. Madhuri, K. B. Chandrasekhar, N. Devanna and G. Somasekhar. (2010) Direct and derivative spectrophotometric determination of gemifloxacin mesylate in pure form and pharmaceutical preparations using p acceptors, Int. J. Pharm. Sci. Res. 1:222–230.
[10] Z. Y. Al Shoaibi and A. A. Gouda. (2010). Spectrophotometric methods for the determination of gemifloxacin mesylate in pure form and pharmaceutical formulations, Anal. Chem: Indian J. 9: 129–136.
[11] M. V. Krishna and D. G. Sankar. (2008). Utility of s and p-acceptors for the spectrophotometric determination of gemifloxacin mesylate in pharmaceutical formulations, E-J. Chem. 5: 493–498.
[12] S. E. K. Tekkeli and A. Önal. (2011). Spectrofluorimetric methods for the determination of gemifloxacin in tablets and spiked plasma samples, J. Fluoresc. 21: 1001–1007.
[13] N. F. Youssef and L. I. Bebawy. (2006). Spectrofluorometric methods for the determination of gemifloxacin mesylate and cefamandole nafate in bulk powder and pharmaceutical preparations, Bull. Fac. Pharm. Cairo Univ. 44: 215–227.
[14] A. A. Elbashir, B. A. Saad, S. M. Abdussalam, K. M. M. Al-Azzam and H. Y. Aboul-Enein. (2008). Validated stability indicating assay of gemifloxacin and lomefloxacin in tablet formulations by capillary electrophoresis, J. Liq. Chromatogr. Rel. Tech. 31: 1465–1477.
[15] A. R. Rote and S. P. Pingle. (2009). Reversed phase-HPLC and HPTLC methods for determination of gemifloxacin mesylate in human plasma, J. Chromatogr. B 877: 3719–3723.
[16] D. Nagavalli, G. Abirami and S. K. Kumar (2011). Validated HPLC method for the simultaneous estimation of gemifloxacin mesylate and ambroxol hydrochloride in bulk and tablet dosage form, J. Pharm. Res. 4:1701–1703.
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    Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. (2017). Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Science Journal of Analytical Chemistry, 5(1), 1-7. https://doi.org/10.11648/j.sjac.20170501.11

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    ACS Style

    Sharifah Ghaythan Dbsan Alharthi; Ayman Abdelaziz Osman; Salma Ali Altamimi; Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci. J. Anal. Chem. 2017, 5(1), 1-7. doi: 10.11648/j.sjac.20170501.11

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    AMA Style

    Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci J Anal Chem. 2017;5(1):1-7. doi: 10.11648/j.sjac.20170501.11

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  • @article{10.11648/j.sjac.20170501.11,
      author = {Sharifah Ghaythan Dbsan Alharthi and Ayman Abdelaziz Osman and Salma Ali Altamimi and Fatma Elzahraa Ali},
      title = {Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine},
      journal = {Science Journal of Analytical Chemistry},
      volume = {5},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.sjac.20170501.11},
      url = {https://doi.org/10.11648/j.sjac.20170501.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20170501.11},
      abstract = {A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.},
     year = {2017}
    }
    

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    T1  - Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine
    AU  - Sharifah Ghaythan Dbsan Alharthi
    AU  - Ayman Abdelaziz Osman
    AU  - Salma Ali Altamimi
    AU  - Fatma Elzahraa Ali
    Y1  - 2017/02/06
    PY  - 2017
    N1  - https://doi.org/10.11648/j.sjac.20170501.11
    DO  - 10.11648/j.sjac.20170501.11
    T2  - Science Journal of Analytical Chemistry
    JF  - Science Journal of Analytical Chemistry
    JO  - Science Journal of Analytical Chemistry
    SP  - 1
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2376-8053
    UR  - https://doi.org/10.11648/j.sjac.20170501.11
    AB  - A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.
    VL  - 5
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia

  • Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia

  • Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

  • Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

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