Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status

Hou Zhenjiang; Mu Zhaoxin; Wang Cuicui; Feng Jing; Chen Yunxia; Ma Jinqun; Liu Chunyan

doi:doi:10.11648/j.sjph.20210903.13

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Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status

Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, Ma Jinqun, Liu Chunyan

Published in Science Journal of Public Health (Volume 9, Issue 3)

Received: 6 April 2021 Published: 24 May 2021

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Abstract

Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (P<0.05), but they had nothing to do with iodine nutrition status. The C/G and C/C genotypes at the PTPN22 gene (1123 G>C) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.

Published in	Science Journal of Public Health (Volume 9, Issue 3)
DOI	10.11648/j.sjph.20210903.13
Page(s)	83-91
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

PTPN22, Gene Polymorphism, Th17/Treg Cells and Factors, Iodine Nutritional Status, Graves' Disease

References

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APA Style

Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. (2021). Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Science Journal of Public Health, 9(3), 83-91. https://doi.org/10.11648/j.sjph.20210903.13

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ACS Style

Hou Zhenjiang; Mu Zhaoxin; Wang Cuicui; Feng Jing; Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(3), 83-91. doi: 10.11648/j.sjph.20210903.13

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AMA Style

Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(3):83-91. doi: 10.11648/j.sjph.20210903.13

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@article{10.11648/j.sjph.20210903.13,
  author = {Hou Zhenjiang and Mu Zhaoxin and Wang Cuicui and Feng Jing and Chen Yunxia and Ma Jinqun and Liu Chunyan},
  title = {Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {3},
  pages = {83-91},
  doi = {10.11648/j.sjph.20210903.13},
  url = {https://doi.org/10.11648/j.sjph.20210903.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210903.13},
  abstract = {Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.},
 year = {2021}
}

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TY  - JOUR
T1  - Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status
AU  - Hou Zhenjiang
AU  - Mu Zhaoxin
AU  - Wang Cuicui
AU  - Feng Jing
AU  - Chen Yunxia
AU  - Ma Jinqun
AU  - Liu Chunyan
Y1  - 2021/05/24
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210903.13
DO  - 10.11648/j.sjph.20210903.13
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 83
EP  - 91
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210903.13
AB  - Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.
VL  - 9
IS  - 3
ER  -

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Author Information

Hou Zhenjiang

Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou Medical College, Cangzhou, China
Mu Zhaoxin

Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou Medical College, Cangzhou, China
Wang Cuicui

Department of Medical Technology, Cangzhou Medical College, Cangzhou, China
Feng Jing

Cangzhou Central Hospital, Cangzhou, China
Chen Yunxia

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Ma Jinqun

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Liu Chunyan

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China

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Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. (2021). Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Science Journal of Public Health, 9(3), 83-91. https://doi.org/10.11648/j.sjph.20210903.13

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ACS Style

Hou Zhenjiang; Mu Zhaoxin; Wang Cuicui; Feng Jing; Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci. J. Public Health 2021, 9(3), 83-91. doi: 10.11648/j.sjph.20210903.13

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AMA Style

Hou Zhenjiang, Mu Zhaoxin, Wang Cuicui, Feng Jing, Chen Yunxia, et al. Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status. Sci J Public Health. 2021;9(3):83-91. doi: 10.11648/j.sjph.20210903.13

Copy | Download

@article{10.11648/j.sjph.20210903.13,
  author = {Hou Zhenjiang and Mu Zhaoxin and Wang Cuicui and Feng Jing and Chen Yunxia and Ma Jinqun and Liu Chunyan},
  title = {Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {3},
  pages = {83-91},
  doi = {10.11648/j.sjph.20210903.13},
  url = {https://doi.org/10.11648/j.sjph.20210903.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210903.13},
  abstract = {Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.},
 year = {2021}
}

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TY  - JOUR
T1  - Correlation Analysis of PTPN22 Gene Polymorphism, Th17 / Treg Cells and Their Cytokines in GD with Different Iodine Nutritional Status
AU  - Hou Zhenjiang
AU  - Mu Zhaoxin
AU  - Wang Cuicui
AU  - Feng Jing
AU  - Chen Yunxia
AU  - Ma Jinqun
AU  - Liu Chunyan
Y1  - 2021/05/24
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210903.13
DO  - 10.11648/j.sjph.20210903.13
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 83
EP  - 91
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210903.13
AB  - Background: PTPN22 is an important candidate gene for autoimmune diseases and its SNP is involved in the pathogenesis of a variety of autoimmune diseases. Objective: To investigate the relationship between the PTPN22 gene polymorphism (rs2488457) and the Th17 and Treg cells and factors in the peripheral blood mononuclear cells (PBMC) of Graves' disease (GD) patients with different iodine nutrition conditions. Methods: We selected 100 GD patients and 60 healthy people in Cangzhou Central Hospital and People's Hospital from September 2019 to August 2020 and used SASP-PCR technology to calculate the PTPN22-1123 genotype and allele frequency. Flow cytometrywere used to detect the ratio and ratio of Th17 and Treg cells in PBMC and real-time PCR were used to detect the mRNA expression levels of ROR-γt and Foxp3. ELISA method was used to detect serum IL-17 and TGF-β levels. Results: The PTPN22 gene -1123G>C (rs2488457) of GD patients has a certain correlation with Th17 and Treg cells and their factors in PBMC. The distribution frequencies of various genotypes and alleles were statistically different between GD patients and the control group (PC) of may increase the risk of GD, while the G/G genotype may reduce the risk of GD. Conclusion: PTPN22 gene polymorphism (rs2488457) in GD patients and Th17 and Treg cells and their factors in PBMC are related to GD susceptibility.
VL  - 9
IS  - 3
ER  -

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