Research Article
Performance Evaluation of the HemoTypeSCTM Rapid Screening Test for the Detection of Hemoglobin A, S, and C Variants at the Fann National University Hospital
Sene Moustapha Ndep*
,
Kandji Pape Matar,
Barry Nene Oumou Kesso,
Thioune Ndeye Mareme,
Ndiaye Siny,
Djite Moustapha,
Ndour El Malick,
Gueye-Tall Fatou,
Gueye Papa Madieye
Issue:
Volume 14, Issue 4, December 2026
Pages:
89-93
Received:
7 September 2026
Accepted:
20 September 2026
Published:
23 October 2026
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of the HemoTypeSCTM rapid test for detecting hemoglobin A, S, and C variants, in accordance with the NF EN ISO 15189 standard. A prospective analytical study was conducted at the Biochemistry-Hematology Laboratory of Fann National University Hospital Center. Patients referred for hemoglobin electrophoresis were tested using HemoTypeSCTM, with the Emmel test and capillary electrophoresis (Capillarys 3 octa, Sebia) as comparative methods. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall agreement were calculated with 95% confidence intervals. Among the 126 samples, 19 had normal profiles and 107 had hemoglobinopathies. Compared with the Emmel test, HemoTypeSCTM showed 100% sensitivity and specificity. Compared with capillary electrophoresis, sensitivity was 98.16%, specificity 100%, PPV 100%, and NPV 90.48%. Two discrepancies in fine typing were observed among the 107 abnormal profiles, with no missed abnormal profiles. HemoTypeSCTM is a rapid, reliable, and easy-to-use test for first-line sickle cell disease screening in resource-limited settings. Positive results should be systematically confirmed by electrophoresis.
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of th...
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Research Article
Therapeutic Effects of 80% Methanolic Cannabis Sativa Leaf Extract on Copper (II) Oxide-Induced Hepatorenal Toxicity in Wistar Rats
Issue:
Volume 14, Issue 4, December 2026
Pages:
94-99
Received:
15 August 2026
Accepted:
26 August 2026
Published:
29 October 2026
Abstract: This study aimed to investigate the protective effect of 80% methanolic Cannabis sativa leaf extract against copper (II) oxide (CuO)-induced hepatorenal toxicity in Wistar rats. Acute toxicity studies were conducted to determine the median lethal dose (LD₅₀) of the crude extract and CuO, followed by an in vivo assessment of the protective effect of the extract in rats exposed to CuO. The crude C. sativa leaf extract exhibited relatively low acute toxicity, with an LD₅₀ of 676.08 mg/kg, compared with 45.71 mg/kg for CuO, indicating greater acute toxicity of CuO. CuO exposure produced significant alterations in selected hepatic and renal biochemical parameters compared with the normal control group. Administration of the 80% methanolic C. sativa leaf extract following CuO exposure significantly ameliorated these alterations, with significant differences (P < 0.05) observed among the experimental groups. The observed changes in hepatic and renal biomarkers suggest that CuO exposure may impair normal hepatorenal function, whereas the improvement following extract administration indicates a potential protective effect of C. sativa. These findings suggest that 80% methanolic C. sativa leaf extract possesses ameliorative properties against CuO-induced hepatorenal toxicity in Wistar rats. The extract may therefore represent a potential source of bioactive compounds for mitigating heavy-metal-induced hepatic and renal injury. Further studies are warranted to identify the active phytochemical constituents and elucidate the molecular mechanisms underlying the observed protective effects.
Abstract: This study aimed to investigate the protective effect of 80% methanolic Cannabis sativa leaf extract against copper (II) oxide (CuO)-induced hepatorenal toxicity in Wistar rats. Acute toxicity studies were conducted to determine the median lethal dose (LD₅₀) of the crude extract and CuO, followed by an in vivo assessment of the protective effect of...
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