Kinetic Resolution of (<i>R,S</i>)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor

Fang-Di Cong; Jie Kang; Wu-Dan Bi; Tao Li; Ping Li

doi:doi:10.11648/j.bmb.20160103.12

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Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor

Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li

Published in Biochemistry and Molecular Biology (Volume 1, Issue 3)

Received: 30 October 2016 Accepted: 15 November 2016 Published: 14 December 2016

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Abstract

A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.

Published in	Biochemistry and Molecular Biology (Volume 1, Issue 3)
DOI	10.11648/j.bmb.20160103.12
Page(s)	34-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Lipase, Activity, Enantioselectivity, Immobilization, Pseudomonas Cepacia Lipase, Bioreactor

References

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APA Style

Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li. (2016). Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochemistry and Molecular Biology, 1(3), 34-38. https://doi.org/10.11648/j.bmb.20160103.12

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ACS Style

Fang-Di Cong; Jie Kang; Wu-Dan Bi; Tao Li; Ping Li. Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochem. Mol. Biol. 2016, 1(3), 34-38. doi: 10.11648/j.bmb.20160103.12

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AMA Style

Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li. Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochem Mol Biol. 2016;1(3):34-38. doi: 10.11648/j.bmb.20160103.12

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@article{10.11648/j.bmb.20160103.12,
  author = {Fang-Di Cong and Jie Kang and Wu-Dan Bi and Tao Li and Ping Li},
  title = {Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor},
  journal = {Biochemistry and Molecular Biology},
  volume = {1},
  number = {3},
  pages = {34-38},
  doi = {10.11648/j.bmb.20160103.12},
  url = {https://doi.org/10.11648/j.bmb.20160103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160103.12},
  abstract = {A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.},
 year = {2016}
}

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TY  - JOUR
T1  - Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor
AU  - Fang-Di Cong
AU  - Jie Kang
AU  - Wu-Dan Bi
AU  - Tao Li
AU  - Ping Li
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.bmb.20160103.12
DO  - 10.11648/j.bmb.20160103.12
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 34
EP  - 38
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20160103.12
AB  - A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.
VL  - 1
IS  - 3
ER  -

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Author Information

Fang-Di Cong

Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Jie Kang

Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Wu-Dan Bi

Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Tao Li

Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China
Ping Li

Department of Biopharmaceuticals, College of Basic Sciences, Tianjin Agricultural University, Tianjin, China

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Plain Text BibTeX RIS

APA Style

Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li. (2016). Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochemistry and Molecular Biology, 1(3), 34-38. https://doi.org/10.11648/j.bmb.20160103.12

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ACS Style

Fang-Di Cong; Jie Kang; Wu-Dan Bi; Tao Li; Ping Li. Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochem. Mol. Biol. 2016, 1(3), 34-38. doi: 10.11648/j.bmb.20160103.12

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AMA Style

Fang-Di Cong, Jie Kang, Wu-Dan Bi, Tao Li, Ping Li. Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor. Biochem Mol Biol. 2016;1(3):34-38. doi: 10.11648/j.bmb.20160103.12

Copy | Download

@article{10.11648/j.bmb.20160103.12,
  author = {Fang-Di Cong and Jie Kang and Wu-Dan Bi and Tao Li and Ping Li},
  title = {Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor},
  journal = {Biochemistry and Molecular Biology},
  volume = {1},
  number = {3},
  pages = {34-38},
  doi = {10.11648/j.bmb.20160103.12},
  url = {https://doi.org/10.11648/j.bmb.20160103.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160103.12},
  abstract = {A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Kinetic Resolution of (R,S)-1-chloro-3-(1-naphthyloxy) -2-Propanol in an Immobilized Lipase Bioreactor
AU  - Fang-Di Cong
AU  - Jie Kang
AU  - Wu-Dan Bi
AU  - Tao Li
AU  - Ping Li
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.bmb.20160103.12
DO  - 10.11648/j.bmb.20160103.12
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 34
EP  - 38
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20160103.12
AB  - A bioreactor with Pseudomonas cepacia lipase (PCL) immobilized on the inner wall was conveniently prepared by adding lipase powder and the right amount of water to a conical flask and keeping it with mouth open in an incubator shaker at 37°C and 170 rpm for more than 10 h. The bioreactor was employed on resolution of (R, S)-1-chloro-3-(1-naphthyloxy)-2-propanol by catalyzing transesterification of it with vinyl acetate. It was showed that the wall-PCL behaved an excellent catalytic activity being 10 folds of native PCL in terms of conversion, and high enantioselectivity E = 110. And also the depressed activity of immobilized PCL owing to frequently use in organic phase could be reactivated easily by again shaking bioreactor under the aforesaid conditions after adding water. The enhanced activity was attributed to the simulation on interfacial activation mechanism of lipases at water/oil interface.
VL  - 1
IS  - 3
ER  -

Copy | Download