Insights on Heterologous Expression of Fungal Cellulases in <i>Pichia pastoris</i>

Sajid Kamal; Shahid Ullah Khan; Nawshad Muhammad; Muhammad Shoaib; Mukama Omar; Kaneza Pascal; Mukasekuru Marie Rose; Fubao Fuelbiol Sun

doi:doi:10.11648/j.bmb.20180301.13

Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris

Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, Kaneza Pascal, Mukasekuru Marie Rose, Fubao Fuelbiol Sun

Published in Biochemistry and Molecular Biology (Volume 3, Issue 1)

Received: 17 February 2018 Accepted: 27 March 2018 Published: 2 April 2018

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Abstract

Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.

Published in	Biochemistry and Molecular Biology (Volume 3, Issue 1)
DOI	10.11648/j.bmb.20180301.13
Page(s)	15-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Pichia pastoris, Heterologous Expression, Fungal Cellulases, Promoters, Synthetic Core Promoters

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Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. (2018). Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochemistry and Molecular Biology, 3(1), 15-35. https://doi.org/10.11648/j.bmb.20180301.13

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Sajid Kamal; Shahid Ullah Khan; Nawshad Muhammad; Muhammad Shoaib; Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem. Mol. Biol. 2018, 3(1), 15-35. doi: 10.11648/j.bmb.20180301.13

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Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem Mol Biol. 2018;3(1):15-35. doi: 10.11648/j.bmb.20180301.13

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@article{10.11648/j.bmb.20180301.13,
  author = {Sajid Kamal and Shahid Ullah Khan and Nawshad Muhammad and Muhammad Shoaib and Mukama Omar and Kaneza Pascal and Mukasekuru Marie Rose and Fubao Fuelbiol Sun},
  title = {Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {1},
  pages = {15-35},
  doi = {10.11648/j.bmb.20180301.13},
  url = {https://doi.org/10.11648/j.bmb.20180301.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180301.13},
  abstract = {Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.},
 year = {2018}
}

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TY - JOUR
T1 - Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris
AU - Sajid Kamal
AU - Shahid Ullah Khan
AU - Nawshad Muhammad
AU - Muhammad Shoaib
AU - Mukama Omar
AU - Kaneza Pascal
AU - Mukasekuru Marie Rose
AU - Fubao Fuelbiol Sun
Y1 - 2018/04/02
PY - 2018
N1 - https://doi.org/10.11648/j.bmb.20180301.13
DO - 10.11648/j.bmb.20180301.13
T2 - Biochemistry and Molecular Biology
JF - Biochemistry and Molecular Biology
JO - Biochemistry and Molecular Biology
SP - 15
EP - 35
PB - Science Publishing Group
SN - 2575-5048
UR - https://doi.org/10.11648/j.bmb.20180301.13
AB - Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.
VL - 3
IS - 1
ER -

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Author Information

Sajid Kamal

School of Biotechnology, Jiangnan University, Wuxi, P. R. China
Shahid Ullah Khan

College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan, P. R. China
Nawshad Muhammad

Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology, Lahore, Pakistan
Muhammad Shoaib

School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China
Mukama Omar

Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, P. R. China
Kaneza Pascal

School of Biotechnology, Jiangnan University, Wuxi, P. R. China
Mukasekuru Marie Rose

School of Biotechnology, Jiangnan University, Wuxi, P. R. China
Fubao Fuelbiol Sun

School of Biotechnology, Jiangnan University, Wuxi, P. R. China

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APA Style

Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. (2018). Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochemistry and Molecular Biology, 3(1), 15-35. https://doi.org/10.11648/j.bmb.20180301.13

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ACS Style

Sajid Kamal; Shahid Ullah Khan; Nawshad Muhammad; Muhammad Shoaib; Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem. Mol. Biol. 2018, 3(1), 15-35. doi: 10.11648/j.bmb.20180301.13

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AMA Style

Sajid Kamal, Shahid Ullah Khan, Nawshad Muhammad, Muhammad Shoaib, Mukama Omar, et al. Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris. Biochem Mol Biol. 2018;3(1):15-35. doi: 10.11648/j.bmb.20180301.13

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@article{10.11648/j.bmb.20180301.13,
  author = {Sajid Kamal and Shahid Ullah Khan and Nawshad Muhammad and Muhammad Shoaib and Mukama Omar and Kaneza Pascal and Mukasekuru Marie Rose and Fubao Fuelbiol Sun},
  title = {Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {1},
  pages = {15-35},
  doi = {10.11648/j.bmb.20180301.13},
  url = {https://doi.org/10.11648/j.bmb.20180301.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180301.13},
  abstract = {Cellulosic biomass, the most remarkable renewable source of fuels and other compounds of potential importance, needs highly complex mixture of various enzymes for its degradation. For sustainable and economical bioprocess, the availability of these enzymes in high quantities and at a low price is much warranted. The advancements in discovery of new strains, highly valuable techniques and molecular biotechnological tools have led to Pichia pastoris gaining a recognized organism status both at industrial as well as laboratory level. With numerous beneficial characteristics on its credit P. pastoris has emerged a promising host for most of heterologous proteins production. Thus, the production of fungal cellulytic enzymes is of worth to be comprehensively illustrated. There are some activators including promoters used in the expression host to increase the utility of expression system. This review summarizes the heterologous expression of fungal cellulases (Exo-glucanases, endo-glucanases (EGs) and beta-glucosidases (BGLs)) and advocates the various kinds of promoters used in P. Pastoris for expression of these enzymes and proteins. Further, concluding remarks will provide insights revealing the scope of P. pastoris as a potential expression system tool in today's modern direction of research.},
 year = {2018}
}

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