<i>In vitro</i> Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D<sub>3</sub>

Gary Richard Gerber; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal; Snehasis Jana

doi:doi:10.11648/j.bmb.20180301.14

In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D₃

Gary Richard Gerber, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Published in Biochemistry and Molecular Biology (Volume 3, Issue 1)

Received: 1 February 2018 Accepted: 16 February 2018 Published: 19 April 2018

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Abstract

The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Gary Richard Gerber and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The cell viability assay (MTT) data showed that the test samples were found as safe in all the tested concentrations. The level of ALP was significantly increased by 74.54% and 98.26% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 111.17% in the BT-DMEM + BT-Test item group at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 147.46%, 59.67%, and 35.36% in the UT-DMEM + BT-Test item group at 1, 10, and 50 µg/mL, respectively compared to the untreated group. Moreover, the level of collagen was significantly increased by 89.88%, 86.42%, and 82.08% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Further, the collagen level was significantly increased by 365.76%, 80.10%, and 76.09% in the BT-DMEM + BT-Test item group at 0.1, 10, and 50 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 59.30%, 30.69%, and 177.7% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 29.44%, and 45.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D₃ was remarkably improved the bone health parameters and help to combat vitamin D₃ deficiency and fight against various bone related problems including low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Published in	Biochemistry and Molecular Biology (Volume 3, Issue 1)
DOI	10.11648/j.bmb.20180301.14
Page(s)	36-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

The Trivedi Effect^®, Biofield Energy Healing Treatment, Osteosarcoma Cells (MG-63), Alizarin Red S Staining, Vitamin D₃ Deficiency, Osteoporosis, Low Bone Density

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APA Style

Gary Richard Gerber, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2018). In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochemistry and Molecular Biology, 3(1), 36-44. https://doi.org/10.11648/j.bmb.20180301.14

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ACS Style

Gary Richard Gerber; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochem. Mol. Biol. 2018, 3(1), 36-44. doi: 10.11648/j.bmb.20180301.14

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AMA Style

Gary Richard Gerber, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochem Mol Biol. 2018;3(1):36-44. doi: 10.11648/j.bmb.20180301.14

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@article{10.11648/j.bmb.20180301.14,
  author = {Gary Richard Gerber and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
  title = {In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {1},
  pages = {36-44},
  doi = {10.11648/j.bmb.20180301.14},
  url = {https://doi.org/10.11648/j.bmb.20180301.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180301.14},
  abstract = {The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Gary Richard Gerber and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The cell viability assay (MTT) data showed that the test samples were found as safe in all the tested concentrations. The level of ALP was significantly increased by 74.54% and 98.26% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 111.17% in the BT-DMEM + BT-Test item group at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 147.46%, 59.67%, and 35.36% in the UT-DMEM + BT-Test item group at 1, 10, and 50 µg/mL, respectively compared to the untreated group. Moreover, the level of collagen was significantly increased by 89.88%, 86.42%, and 82.08% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Further, the collagen level was significantly increased by 365.76%, 80.10%, and 76.09% in the BT-DMEM + BT-Test item group at 0.1, 10, and 50 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 59.30%, 30.69%, and 177.7% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 29.44%, and 45.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was remarkably improved the bone health parameters and help to combat vitamin D3 deficiency and fight against various bone related problems including low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.},
 year = {2018}
}

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TY  - JOUR
T1  - In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3
AU  - Gary Richard Gerber
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Sambhu Charan Mondal
AU  - Snehasis Jana
Y1  - 2018/04/19
PY  - 2018
N1  - https://doi.org/10.11648/j.bmb.20180301.14
DO  - 10.11648/j.bmb.20180301.14
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 36
EP  - 44
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180301.14
AB  - The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Gary Richard Gerber and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The cell viability assay (MTT) data showed that the test samples were found as safe in all the tested concentrations. The level of ALP was significantly increased by 74.54% and 98.26% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 111.17% in the BT-DMEM + BT-Test item group at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 147.46%, 59.67%, and 35.36% in the UT-DMEM + BT-Test item group at 1, 10, and 50 µg/mL, respectively compared to the untreated group. Moreover, the level of collagen was significantly increased by 89.88%, 86.42%, and 82.08% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Further, the collagen level was significantly increased by 365.76%, 80.10%, and 76.09% in the BT-DMEM + BT-Test item group at 0.1, 10, and 50 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 59.30%, 30.69%, and 177.7% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 29.44%, and 45.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was remarkably improved the bone health parameters and help to combat vitamin D3 deficiency and fight against various bone related problems including low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
VL  - 3
IS  - 1
ER  -

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Author Information

Gary Richard Gerber

Trivedi Global, Inc., Henderson, Nevada, USA
Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton

Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, Nevada, USA
Sambhu Charan Mondal

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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Gary Richard Gerber, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2018). In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochemistry and Molecular Biology, 3(1), 36-44. https://doi.org/10.11648/j.bmb.20180301.14

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ACS Style

Gary Richard Gerber; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochem. Mol. Biol. 2018, 3(1), 36-44. doi: 10.11648/j.bmb.20180301.14

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AMA Style

Gary Richard Gerber, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3. Biochem Mol Biol. 2018;3(1):36-44. doi: 10.11648/j.bmb.20180301.14

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@article{10.11648/j.bmb.20180301.14,
  author = {Gary Richard Gerber and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
  title = {In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {1},
  pages = {36-44},
  doi = {10.11648/j.bmb.20180301.14},
  url = {https://doi.org/10.11648/j.bmb.20180301.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180301.14},
  abstract = {The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Gary Richard Gerber and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The cell viability assay (MTT) data showed that the test samples were found as safe in all the tested concentrations. The level of ALP was significantly increased by 74.54% and 98.26% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 111.17% in the BT-DMEM + BT-Test item group at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 147.46%, 59.67%, and 35.36% in the UT-DMEM + BT-Test item group at 1, 10, and 50 µg/mL, respectively compared to the untreated group. Moreover, the level of collagen was significantly increased by 89.88%, 86.42%, and 82.08% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Further, the collagen level was significantly increased by 365.76%, 80.10%, and 76.09% in the BT-DMEM + BT-Test item group at 0.1, 10, and 50 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 59.30%, 30.69%, and 177.7% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 29.44%, and 45.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was remarkably improved the bone health parameters and help to combat vitamin D3 deficiency and fight against various bone related problems including low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.},
 year = {2018}
}

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TY  - JOUR
T1  - In vitro Study on Human Bone Osteosarcoma Cells (MG-63): Role of Biofield Energy Treated Vitamin D3
AU  - Gary Richard Gerber
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Sambhu Charan Mondal
AU  - Snehasis Jana
Y1  - 2018/04/19
PY  - 2018
N1  - https://doi.org/10.11648/j.bmb.20180301.14
DO  - 10.11648/j.bmb.20180301.14
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 36
EP  - 44
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180301.14
AB  - The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Gary Richard Gerber and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The cell viability assay (MTT) data showed that the test samples were found as safe in all the tested concentrations. The level of ALP was significantly increased by 74.54% and 98.26% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 111.17% in the BT-DMEM + BT-Test item group at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 147.46%, 59.67%, and 35.36% in the UT-DMEM + BT-Test item group at 1, 10, and 50 µg/mL, respectively compared to the untreated group. Moreover, the level of collagen was significantly increased by 89.88%, 86.42%, and 82.08% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Further, the collagen level was significantly increased by 365.76%, 80.10%, and 76.09% in the BT-DMEM + BT-Test item group at 0.1, 10, and 50 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 59.30%, 30.69%, and 177.7% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 29.44%, and 45.51% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was remarkably improved the bone health parameters and help to combat vitamin D3 deficiency and fight against various bone related problems including low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
VL  - 3
IS  - 1
ER  -

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