Role of Polymorphism in P<i>LA2G7</i> Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India

Nazia; Tehseen Hassan; Showkat Ahmad Bhat; Ishraq Hussain; Muneeb U Rehman; Sabhiya Majid; Roohi Ashraf; Sheikh Bilal Ahmad

doi:doi:10.11648/j.ccr.20170102.16

Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India

Nazia, Tehseen Hassan, Showkat Ahmad Bhat, Ishraq Hussain, Muneeb U Rehman, Sabhiya Majid, Roohi Ashraf, Sheikh Bilal Ahmad

Published in Cardiology and Cardiovascular Research (Volume 1, Issue 2)

Received: 29 April 2017 Accepted: 6 May 2017 Published: 15 June 2017

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Abstract

Background: Coronary artery disease (CAD) also known as atherosclerotic heart disease. Atherosclerosis is a multifactorial progressive disease manifested by the focal development within the arterial wall of lesions the atherosclerotic plaques. The PLA2G7 gene polymorphism is related to enzyme activity which is related to the pro atherosclerotic abilities. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD) and healthy subjects. Material and method: In this study, the PLA2G7 genotype of 30 patients with coronary artery disease, and 20 healthy subjects were assessed by Polymerase chain reaction polymorphism (PCR-RFLP) method. On genetic level the role of polymorphism of PLA2G7 gene has been widely studied across the world in different ethnic populations. Results: Our study shown 66% (20/30) of the Coronary Heart Disease cases showed homozygous and 26% (8/30) of the cases however showed heterozygous condition. Almost all 80% (16/20) of the normal samples showed heterozygous condition except in four cases where PLA2G7 gene was found to be Homozygous Mutant two & Homozygous normal two. The allelic association of this polymorphism with Coronary Heart Disease was evaluated by χ² (Chi square) test and was found to be significant (P=0.006).Homozygous mutant condition of PLA2G7 gene was found to be certainly higher in Coronary Heart Disease Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Conclusion: Our study suggests that the polymorphisms of PLA2G7 gene act synergistically to increase the risk of Coronary Heart Disease. Furthermore, it should be noted that the sample size was relatively small in the studied population and so large-scale prospective studies are needed to conﬁrm these ﬁndings.

Published in	Cardiology and Cardiovascular Research (Volume 1, Issue 2)
DOI	10.11648/j.ccr.20170102.16
Page(s)	62-66
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Coronary Artery Disease, Heterozygous, Homozygous & PLA2G7 Gene

References

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[2]	Beisiegel U, Weber W, Bengtsson-Oliveecrono G. Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein. ProcNatlAcadSci USA 1991; 88: 8342–6.
[3]	Fisher RM, Humphries SE, Talmud PJ. Common variation in the lipoprotein lipase gene: effects of plasma lipids and atherosclerosis. Atherosclerosis 1997; 13: 145–59.
[4]	Beisiegel U, Weber W, Bengtsson-Oliveecrono G. Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein. ProcNatlAcadSci USA 1991; 88: 8342–6.
[5]	Hokanson JE, Austin MA. Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of pop-ulation-based prospective studies. J Cardiovasc Risk 1996; 3: 213–9.
[6]	Sandhu MS, Waterworth DM, Debenham SL, et al: LDL-cholesterol concentrations: a genome-wide association study. Lancet 2008: 371: 483-491.
[7]	Doll R, Hill AB. Mortality of British doctors in relation to smoking; observation on coronary thrombosis. In: Haenzel W, ed. Epedemiological Approaches to the Study of Cancer and Other Chronic Disease. National Cancer Institute Monograph No. 19, Bethesda, MD, USA: US Department of Health, Education and Welfare, 1966; 99: 205–68.
[8]	Kruth HS Localization of unesterified cholesterol in human atherosclerotic lesions.Demonstration of filipinpositive, Oil-Red-0-negative particles. Am J Pathol 1984: 114:201–8.
[9]	Millonig G, Malcom GT, Wick G Early inflammatory-immunological lesions injuvenile atherosclerosis from the pathobiological determinants of atherosclerosis in youth (PDAY)-study. Atherosclerosis 2002; 160:441–448.
[10]	Ping-Yen Liu, Jing-Hong Chen, Y-H. Lit, GY Shi. W02-P- 008 synergistic effect of stromelysi-promotor 5A/6A polymorphism on the onset of acutecoronary syndrome. Atherosclerosis Supplements: 2005; 6: 9-9.
[11]	Jiang D, Zheng D, Wang L, Huang Y, Liu H, XU L. Elevated PLA2G7 gene as gender specific of aging increases the risk of coronary heart disease in females. PLOS ONE; 2012; 8:3:e59752.
[12]	MC T. M, Prescot S,M, Stafforini D.M. The emerging role of PAF Acetylhydrolase. J. lipid Res. 2009; 50: S255-S259.
[13]	Zheng GH, Xiong SQ, Mei LJ, Chen HY, Wang T. Elevated plasma platlet activating factor levels and risk of coronary heart disease or blood stasis syndrome of coronary heart diseasein Chinese; Inflammation: 2012; 35:4: 1419-28.

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Nazia, Tehseen Hassan, Showkat Ahmad Bhat, Ishraq Hussain, Muneeb U Rehman, et al. (2017). Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiology and Cardiovascular Research, 1(2), 62-66. https://doi.org/10.11648/j.ccr.20170102.16

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ACS Style

Nazia; Tehseen Hassan; Showkat Ahmad Bhat; Ishraq Hussain; Muneeb U Rehman, et al. Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiol. Cardiovasc. Res. 2017, 1(2), 62-66. doi: 10.11648/j.ccr.20170102.16

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AMA Style

Nazia, Tehseen Hassan, Showkat Ahmad Bhat, Ishraq Hussain, Muneeb U Rehman, et al. Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiol Cardiovasc Res. 2017;1(2):62-66. doi: 10.11648/j.ccr.20170102.16

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@article{10.11648/j.ccr.20170102.16,
  author = {Nazia and Tehseen Hassan and Showkat Ahmad Bhat and Ishraq Hussain and Muneeb U Rehman and Sabhiya Majid and Roohi Ashraf and Sheikh Bilal Ahmad},
  title = {Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India},
  journal = {Cardiology and Cardiovascular Research},
  volume = {1},
  number = {2},
  pages = {62-66},
  doi = {10.11648/j.ccr.20170102.16},
  url = {https://doi.org/10.11648/j.ccr.20170102.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ccr.20170102.16},
  abstract = {Background: Coronary artery disease (CAD) also known as atherosclerotic heart disease. Atherosclerosis is a multifactorial progressive disease manifested by the focal development within the arterial wall of lesions the atherosclerotic plaques. The PLA2G7 gene polymorphism is related to enzyme activity which is related to the pro atherosclerotic abilities. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD) and healthy subjects. Material and method: In this study, the PLA2G7 genotype of 30 patients with coronary artery disease, and 20 healthy subjects were assessed by Polymerase chain reaction polymorphism (PCR-RFLP) method. On genetic level the role of polymorphism of PLA2G7 gene has been widely studied across the world in different ethnic populations. Results: Our study shown 66% (20/30) of the Coronary Heart Disease cases showed homozygous and 26% (8/30) of the cases however showed heterozygous condition. Almost all 80% (16/20) of the normal samples showed heterozygous condition except in four cases where PLA2G7 gene was found to be Homozygous Mutant two & Homozygous normal two. The allelic association of this polymorphism with Coronary Heart Disease was evaluated by χ2 (Chi square) test and was found to be significant (P=0.006).Homozygous mutant condition of PLA2G7 gene was found to be certainly higher in Coronary Heart Disease Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Conclusion: Our study suggests that the polymorphisms of PLA2G7 gene act synergistically to increase the risk of Coronary Heart Disease. Furthermore, it should be noted that the sample size was relatively small in the studied population and so large-scale prospective studies are needed to conﬁrm these ﬁndings.},
 year = {2017}
}

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TY  - JOUR
T1  - Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India
AU  - Nazia
AU  - Tehseen Hassan
AU  - Showkat Ahmad Bhat
AU  - Ishraq Hussain
AU  - Muneeb U Rehman
AU  - Sabhiya Majid
AU  - Roohi Ashraf
AU  - Sheikh Bilal Ahmad
Y1  - 2017/06/15
PY  - 2017
N1  - https://doi.org/10.11648/j.ccr.20170102.16
DO  - 10.11648/j.ccr.20170102.16
T2  - Cardiology and Cardiovascular Research
JF  - Cardiology and Cardiovascular Research
JO  - Cardiology and Cardiovascular Research
SP  - 62
EP  - 66
PB  - Science Publishing Group
SN  - 2578-8914
UR  - https://doi.org/10.11648/j.ccr.20170102.16
AB  - Background: Coronary artery disease (CAD) also known as atherosclerotic heart disease. Atherosclerosis is a multifactorial progressive disease manifested by the focal development within the arterial wall of lesions the atherosclerotic plaques. The PLA2G7 gene polymorphism is related to enzyme activity which is related to the pro atherosclerotic abilities. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD) and healthy subjects. Material and method: In this study, the PLA2G7 genotype of 30 patients with coronary artery disease, and 20 healthy subjects were assessed by Polymerase chain reaction polymorphism (PCR-RFLP) method. On genetic level the role of polymorphism of PLA2G7 gene has been widely studied across the world in different ethnic populations. Results: Our study shown 66% (20/30) of the Coronary Heart Disease cases showed homozygous and 26% (8/30) of the cases however showed heterozygous condition. Almost all 80% (16/20) of the normal samples showed heterozygous condition except in four cases where PLA2G7 gene was found to be Homozygous Mutant two & Homozygous normal two. The allelic association of this polymorphism with Coronary Heart Disease was evaluated by χ2 (Chi square) test and was found to be significant (P=0.006).Homozygous mutant condition of PLA2G7 gene was found to be certainly higher in Coronary Heart Disease Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Conclusion: Our study suggests that the polymorphisms of PLA2G7 gene act synergistically to increase the risk of Coronary Heart Disease. Furthermore, it should be noted that the sample size was relatively small in the studied population and so large-scale prospective studies are needed to conﬁrm these ﬁndings.
VL  - 1
IS  - 2
ER  -

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Author Information

Nazia

Clinical-Biochemistry University of Kashmir, Hazratbal Srinagar, India
Tehseen Hassan

Department of Biochemistry Govt. Medical College Srinagar, Srinagar, India
Showkat Ahmad Bhat

Department of Biochemistry Govt. Medical College Srinagar, Srinagar, India
Ishraq Hussain

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Muneeb U Rehman

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Sabhiya Majid

Department of Biochemistry Govt. Medical College Srinagar, Srinagar, India
Roohi Ashraf

Department of Biochemistry Govt. Medical College Srinagar, Srinagar, India
Sheikh Bilal Ahmad

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

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Plain Text BibTeX RIS

APA Style

Nazia, Tehseen Hassan, Showkat Ahmad Bhat, Ishraq Hussain, Muneeb U Rehman, et al. (2017). Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiology and Cardiovascular Research, 1(2), 62-66. https://doi.org/10.11648/j.ccr.20170102.16

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ACS Style

Nazia; Tehseen Hassan; Showkat Ahmad Bhat; Ishraq Hussain; Muneeb U Rehman, et al. Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiol. Cardiovasc. Res. 2017, 1(2), 62-66. doi: 10.11648/j.ccr.20170102.16

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AMA Style

Nazia, Tehseen Hassan, Showkat Ahmad Bhat, Ishraq Hussain, Muneeb U Rehman, et al. Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India. Cardiol Cardiovasc Res. 2017;1(2):62-66. doi: 10.11648/j.ccr.20170102.16

Copy | Download

@article{10.11648/j.ccr.20170102.16,
  author = {Nazia and Tehseen Hassan and Showkat Ahmad Bhat and Ishraq Hussain and Muneeb U Rehman and Sabhiya Majid and Roohi Ashraf and Sheikh Bilal Ahmad},
  title = {Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India},
  journal = {Cardiology and Cardiovascular Research},
  volume = {1},
  number = {2},
  pages = {62-66},
  doi = {10.11648/j.ccr.20170102.16},
  url = {https://doi.org/10.11648/j.ccr.20170102.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ccr.20170102.16},
  abstract = {Background: Coronary artery disease (CAD) also known as atherosclerotic heart disease. Atherosclerosis is a multifactorial progressive disease manifested by the focal development within the arterial wall of lesions the atherosclerotic plaques. The PLA2G7 gene polymorphism is related to enzyme activity which is related to the pro atherosclerotic abilities. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD) and healthy subjects. Material and method: In this study, the PLA2G7 genotype of 30 patients with coronary artery disease, and 20 healthy subjects were assessed by Polymerase chain reaction polymorphism (PCR-RFLP) method. On genetic level the role of polymorphism of PLA2G7 gene has been widely studied across the world in different ethnic populations. Results: Our study shown 66% (20/30) of the Coronary Heart Disease cases showed homozygous and 26% (8/30) of the cases however showed heterozygous condition. Almost all 80% (16/20) of the normal samples showed heterozygous condition except in four cases where PLA2G7 gene was found to be Homozygous Mutant two & Homozygous normal two. The allelic association of this polymorphism with Coronary Heart Disease was evaluated by χ2 (Chi square) test and was found to be significant (P=0.006).Homozygous mutant condition of PLA2G7 gene was found to be certainly higher in Coronary Heart Disease Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Conclusion: Our study suggests that the polymorphisms of PLA2G7 gene act synergistically to increase the risk of Coronary Heart Disease. Furthermore, it should be noted that the sample size was relatively small in the studied population and so large-scale prospective studies are needed to conﬁrm these ﬁndings.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Role of Polymorphism in PLA2G7 Gene in Pathogenesis of Atherosclerosis in Patients of Kashmir Valley India
AU  - Nazia
AU  - Tehseen Hassan
AU  - Showkat Ahmad Bhat
AU  - Ishraq Hussain
AU  - Muneeb U Rehman
AU  - Sabhiya Majid
AU  - Roohi Ashraf
AU  - Sheikh Bilal Ahmad
Y1  - 2017/06/15
PY  - 2017
N1  - https://doi.org/10.11648/j.ccr.20170102.16
DO  - 10.11648/j.ccr.20170102.16
T2  - Cardiology and Cardiovascular Research
JF  - Cardiology and Cardiovascular Research
JO  - Cardiology and Cardiovascular Research
SP  - 62
EP  - 66
PB  - Science Publishing Group
SN  - 2578-8914
UR  - https://doi.org/10.11648/j.ccr.20170102.16
AB  - Background: Coronary artery disease (CAD) also known as atherosclerotic heart disease. Atherosclerosis is a multifactorial progressive disease manifested by the focal development within the arterial wall of lesions the atherosclerotic plaques. The PLA2G7 gene polymorphism is related to enzyme activity which is related to the pro atherosclerotic abilities. This study was designed to investigate an association of this polymorphism with coronary artery disease (CAD) and healthy subjects. Material and method: In this study, the PLA2G7 genotype of 30 patients with coronary artery disease, and 20 healthy subjects were assessed by Polymerase chain reaction polymorphism (PCR-RFLP) method. On genetic level the role of polymorphism of PLA2G7 gene has been widely studied across the world in different ethnic populations. Results: Our study shown 66% (20/30) of the Coronary Heart Disease cases showed homozygous and 26% (8/30) of the cases however showed heterozygous condition. Almost all 80% (16/20) of the normal samples showed heterozygous condition except in four cases where PLA2G7 gene was found to be Homozygous Mutant two & Homozygous normal two. The allelic association of this polymorphism with Coronary Heart Disease was evaluated by χ2 (Chi square) test and was found to be significant (P=0.006).Homozygous mutant condition of PLA2G7 gene was found to be certainly higher in Coronary Heart Disease Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Conclusion: Our study suggests that the polymorphisms of PLA2G7 gene act synergistically to increase the risk of Coronary Heart Disease. Furthermore, it should be noted that the sample size was relatively small in the studied population and so large-scale prospective studies are needed to conﬁrm these ﬁndings.
VL  - 1
IS  - 2
ER  -

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