The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin

Lihua Ma; Baosheng Liu; Chundan Wang; Hongcai Zhang; Xu Cheng

doi:doi:10.11648/j.jcebe.20180201.13

| Peer-Reviewed

The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin

Lihua Ma, Baosheng Liu, Chundan Wang, Hongcai Zhang, Xu Cheng

Published in Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 1)

Received: 4 May 2018 Accepted: 30 May 2018 Published: 1 July 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H3L²⁺ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H₂L⁺ form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H₂L⁺ loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of L^- which is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.

Published in	Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 1)
DOI	10.11648/j.jcebe.20180201.13
Page(s)	11-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Enrofloxacin, Fluorescence Spectroscopy, Fluorescence Quantum Yield, Dissociation Constant

References

[1]	Wu Y C, Guo S, Dong Q, Song Y Z. Development of an immunochromatographic test strip for rapid simultaneous detection of enrofloxacin and ofloxacin in tissue of chicken muscle and pork. Food Analytical Methods 2016; 9 (10): 2807-2813.
[2]	Anirudhan T S, Shainy F, Christa J. Synthesis and characterization of polyacrylic acid- grafted-carboxylic graphene/titanium nanotube composite for the effective removal of enrofloxacin from aqueous solutions: Adsorption and photocatalytic degradation studies. Journal of Hazardous Materials 2017; 324(Pt B): 117-130.
[3]	Ftouni H, Sayen S, Boudesocque S, Dechamps-Olivier I, Guillon E, Structural study of the copper (II)–enrofloxacin metallo-antibiotic. Inorganica Chimica Acta 2012; 382(5): 186-190.
[4]	Terrado-Campos D, Tayeb-Cherif K, Peris-Vicente J, Carda-Broch S, Esteve-Romero J. Determination of oxolinic acid, danofloxacin, ciprofloxacin, and enrofloxacin in porcine and bovine meat by micellar liquid chromatography with fluorescence detection. Food chemistry 2017; 221:1277-1284.
[5]	Yan W, Hu S, Jing C Y. Enrofloxacin sorption on smectite clays: Effects of pH, cations, and humic acid, Journal of Colloid and Interface Science 2012; 372(1): 141-147.
[6]	Zhang W S, Tang B, Liu X, Liu Y Y, Xu K H, Ma J P et al. A highly sensitive acidic pH fluorescent probe and its application to HepG2 cells. Analyst 2009; 134(2): 367-371.
[7]	Wierzchowski J, Sepioł J, Sulikowski D, Kierdaszuk B, Shugar D. Fluorescence emission properties of 8-azaxanthine and its N-alkyl derivatives: Excited-state proton transfer, and potential applications in enzymology. Journal of Photochemistry and Photobiology A: Chemistry 2006; 179(3): 276-282.
[8]	Singh R B, Mahanta S, Kar S, Guchhait N. Photo-physical properties of 1-hydroxy-2-naphthaldehyde: A combined fluorescence spectroscopy and quantum chemical calculations. Chemical Physics 2006; 331(2): 373-384.
[9]	Fedosov S N, Fedosova N U, Krutler B, Nex E, Petersen T E. Mechanisms of discrimination between cobalamins and their natural analogues during their binding to the specific B12-transporting proteins. Biochemistry 2007; 46(21): 6446-6458.
[10]	Park H R, Kim T H, Bark K M, Physicochemical properties of quinolone antibiotics in various environments. European Journal of Medicinal Chemistry 2002; 37(6): 443-460.
[11]	Mack J., Vogel P., Jones D., Kaval N., Sutton A., The development of corannulene-based blue emitters. Organic & Biomolecular Chemistry 2007; 5(15): 2448-2452.
[12]	Zhang J, Liu C G., Wei Y J. Fluorescence Quantum Yield and Ionization Constant of Umbelliferone. Chemistry 2011; 74(10): 957-960.
[13]	Tian H. J., Tang R. R., Zhao M. M., Synthesis, characterization, and photophysical properties of dinuclear Eu (III) and Tb (III) complexes based on β-diketonate with triphenylamine and pyridine moieties. ECS Journal of Solid State Science and Technology 2013; 2(3): R33-R38.
[14]	Suzuki K, Kobayashi A, Kaneko S, Takehira K, Yoshihara T, Ishida H et al. Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector, Physical Chemistry Chemical Physics 2009; 11(42): 9850-9860.
[15]	Liu C G, Xu Y Z, Wei Y J, Qi J, Xu Z H, Ye F et al. Fluorescence spectra and protonation of ofloxacin. Spectroscopy and Spectral Analysis 2005; 25(4): 584-587.
[16]	Shelar D P, Rote R V, Patil S R., Jachak M N. Effects of homogeneous media, binary mixtures and microheterogeneous media on the fluorescence and fluorescence probe properties of some benzo [b] [1, 8] naphthyridiens with HSA and BSA. Luminescence 2012; 27(5): 398-413.
[17]	Drobnik J, Yeargers E. On the use of quinine sulfate as a fluorescence standard. Journal of Molecular Spectroscopy 1996; 19(1-4): 454-455.
[18]	Liang X R, Wang G, Jiang Y L, Qu C L, Wang X J, Zhao B. Synthesis and theoretical study on fluorescence property of 4-(2-Hydroxybenzylideneamino)phenyl ethanone schiff base. Spectroscopy and Spectral Analysis 2013; 33(12): 3259-3262.

Cite This Article

Plain Text BibTeX RIS

APA Style

Lihua Ma, Baosheng Liu, Chundan Wang, Hongcai Zhang, Xu Cheng. (2018). The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. Journal of Chemical, Environmental and Biological Engineering, 2(1), 11-16. https://doi.org/10.11648/j.jcebe.20180201.13

Copy | Download

ACS Style

Lihua Ma; Baosheng Liu; Chundan Wang; Hongcai Zhang; Xu Cheng. The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. J. Chem. Environ. Biol. Eng. 2018, 2(1), 11-16. doi: 10.11648/j.jcebe.20180201.13

Copy | Download

AMA Style

Lihua Ma, Baosheng Liu, Chundan Wang, Hongcai Zhang, Xu Cheng. The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. J Chem Environ Biol Eng. 2018;2(1):11-16. doi: 10.11648/j.jcebe.20180201.13

Copy | Download

@article{10.11648/j.jcebe.20180201.13,
  author = {Lihua Ma and Baosheng Liu and Chundan Wang and Hongcai Zhang and Xu Cheng},
  title = {The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {1},
  pages = {11-16},
  doi = {10.11648/j.jcebe.20180201.13},
  url = {https://doi.org/10.11648/j.jcebe.20180201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180201.13},
  abstract = {In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H3L2+ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H2L+ form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H2L+ loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of L- which is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin
AU  - Lihua Ma
AU  - Baosheng Liu
AU  - Chundan Wang
AU  - Hongcai Zhang
AU  - Xu Cheng
Y1  - 2018/07/01
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180201.13
DO  - 10.11648/j.jcebe.20180201.13
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180201.13
AB  - In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H3L2+ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H2L+ form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H2L+ loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of L- which is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.
VL  - 2
IS  - 1
ER  -

Copy | Download

Author Information

Lihua Ma

College of Chemistry and Environmental Science, Hebei University, Baoding, China
Baosheng Liu

College of Chemistry and Environmental Science, Hebei University, Baoding, China
Chundan Wang

College of Chemistry and Environmental Science, Hebei University, Baoding, China
Hongcai Zhang

College of Chemistry and Environmental Science, Hebei University, Baoding, China
Xu Cheng

College of Chemistry and Environmental Science, Hebei University, Baoding, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Lihua Ma, Baosheng Liu, Chundan Wang, Hongcai Zhang, Xu Cheng. (2018). The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. Journal of Chemical, Environmental and Biological Engineering, 2(1), 11-16. https://doi.org/10.11648/j.jcebe.20180201.13

Copy | Download

ACS Style

Lihua Ma; Baosheng Liu; Chundan Wang; Hongcai Zhang; Xu Cheng. The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. J. Chem. Environ. Biol. Eng. 2018, 2(1), 11-16. doi: 10.11648/j.jcebe.20180201.13

Copy | Download

AMA Style

Lihua Ma, Baosheng Liu, Chundan Wang, Hongcai Zhang, Xu Cheng. The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin. J Chem Environ Biol Eng. 2018;2(1):11-16. doi: 10.11648/j.jcebe.20180201.13

Copy | Download

@article{10.11648/j.jcebe.20180201.13,
  author = {Lihua Ma and Baosheng Liu and Chundan Wang and Hongcai Zhang and Xu Cheng},
  title = {The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {1},
  pages = {11-16},
  doi = {10.11648/j.jcebe.20180201.13},
  url = {https://doi.org/10.11648/j.jcebe.20180201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180201.13},
  abstract = {In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H3L2+ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H2L+ form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H2L+ loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of L- which is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin
AU  - Lihua Ma
AU  - Baosheng Liu
AU  - Chundan Wang
AU  - Hongcai Zhang
AU  - Xu Cheng
Y1  - 2018/07/01
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180201.13
DO  - 10.11648/j.jcebe.20180201.13
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180201.13
AB  - In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H3L2+ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H2L+ form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H2L+ loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of L- which is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.
VL  - 2
IS  - 1
ER  -

Copy | Download