Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile
Bai Yanrong,
Wang Jinying
Issue:
Volume 3, Issue 1, March 2020
Pages:
1-6
Received:
7 January 2020
Accepted:
9 June 2020
Published:
28 July 2020
Abstract: Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.
Abstract: Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results s...
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