A microbial composition study of sediments of contaminated (CWS) and uncontaminated (UWS) lentic ecosystems within Akwa Ibom State was carried out by analyzing the small-subunit rRNA genes to determine the effect of hydrocarbon contamination on its microbial composition and diversity. Analysis of the V4 region of the community DNA from both sediments revealed the presence of bacteria, archaea and microalgae. Bacterial sequences outnumbered archaea and microalgae. Abundance of Proteobacteria, Betaproteobacteria, Burholderiales, Alcaligenaceae, and Achrombacter were observed in the CWS and Actinobacteria, Actinomycetales, Bacillaceae, and Bacillus in the UWS. Crenarchaeota and Euryarchaeota were also observed to be present in both sediments. The genus Achromobacter and Bacillus dominated in the CWS and UWS, respectively. Uncultured bacterium with the accession number DQ404672.1 and AY917600.1 led at the species levels. Achromobacter sp.-AM232721.1 outnumbered the other species in the CWS such as Kitasatospora sp.-AF131379.1, Mycobacterium celatum-AF547908.1, Paenibacillus phyllosphaerae-NR_043008.1, Cystobacter fuscus-M94276.1, Planosporangium flavigriseum-NR_042508.1, etc. In the UWS, the dominant species was Bacillus sp.-AJ316313.1. Microalgae, Chlorella sp. and Chlorella vulgaris were also detected in both ecosystems. Diverse and distinct diversity of bacteria, archaea and microalgae are present in the sediments and only a few of them have cultured counterpart. The variation in the microbial communities from the two sites has revealed the impact of contaminants especially hydrocarbons on the microbial diversity in lentic ecosystems.
| Published in | Journal of Chemical, Environmental and Biological Engineering (Volume 4, Issue 2) |
| DOI | 10.11648/j.jcebe.20200402.11 |
| Page(s) | 32-38 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Freshwater, Sediment, Hydrocarbon, Prokaryotes, Microalgae, Culture-Independent
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APA Style
Mfoniso Uko, Ime Udotong, Utibe Ofon, Senyene Umana, Nsikak Abraham. (2020). Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria. Journal of Chemical, Environmental and Biological Engineering, 4(2), 32-38. https://doi.org/10.11648/j.jcebe.20200402.11
ACS Style
Mfoniso Uko; Ime Udotong; Utibe Ofon; Senyene Umana; Nsikak Abraham. Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria. J. Chem. Environ. Biol. Eng. 2020, 4(2), 32-38. doi: 10.11648/j.jcebe.20200402.11
AMA Style
Mfoniso Uko, Ime Udotong, Utibe Ofon, Senyene Umana, Nsikak Abraham. Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria. J Chem Environ Biol Eng. 2020;4(2):32-38. doi: 10.11648/j.jcebe.20200402.11
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Download@article{10.11648/j.jcebe.20200402.11,
author = {Mfoniso Uko and Ime Udotong and Utibe Ofon and Senyene Umana and Nsikak Abraham},
title = {Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria},
journal = {Journal of Chemical, Environmental and Biological Engineering},
volume = {4},
number = {2},
pages = {32-38},
doi = {10.11648/j.jcebe.20200402.11},
url = {https://doi.org/10.11648/j.jcebe.20200402.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20200402.11},
abstract = {A microbial composition study of sediments of contaminated (CWS) and uncontaminated (UWS) lentic ecosystems within Akwa Ibom State was carried out by analyzing the small-subunit rRNA genes to determine the effect of hydrocarbon contamination on its microbial composition and diversity. Analysis of the V4 region of the community DNA from both sediments revealed the presence of bacteria, archaea and microalgae. Bacterial sequences outnumbered archaea and microalgae. Abundance of Proteobacteria, Betaproteobacteria, Burholderiales, Alcaligenaceae, and Achrombacter were observed in the CWS and Actinobacteria, Actinomycetales, Bacillaceae, and Bacillus in the UWS. Crenarchaeota and Euryarchaeota were also observed to be present in both sediments. The genus Achromobacter and Bacillus dominated in the CWS and UWS, respectively. Uncultured bacterium with the accession number DQ404672.1 and AY917600.1 led at the species levels. Achromobacter sp.-AM232721.1 outnumbered the other species in the CWS such as Kitasatospora sp.-AF131379.1, Mycobacterium celatum-AF547908.1, Paenibacillus phyllosphaerae-NR_043008.1, Cystobacter fuscus-M94276.1, Planosporangium flavigriseum-NR_042508.1, etc. In the UWS, the dominant species was Bacillus sp.-AJ316313.1. Microalgae, Chlorella sp. and Chlorella vulgaris were also detected in both ecosystems. Diverse and distinct diversity of bacteria, archaea and microalgae are present in the sediments and only a few of them have cultured counterpart. The variation in the microbial communities from the two sites has revealed the impact of contaminants especially hydrocarbons on the microbial diversity in lentic ecosystems.},
year = {2020}
}
TY - JOUR T1 - Effect of Hydrocarbon Contamination on the Microbial Diversity of Freshwater Sediments Within Akwa Ibom State, Nigeria AU - Mfoniso Uko AU - Ime Udotong AU - Utibe Ofon AU - Senyene Umana AU - Nsikak Abraham Y1 - 2020/06/08 PY - 2020 N1 - https://doi.org/10.11648/j.jcebe.20200402.11 DO - 10.11648/j.jcebe.20200402.11 T2 - Journal of Chemical, Environmental and Biological Engineering JF - Journal of Chemical, Environmental and Biological Engineering JO - Journal of Chemical, Environmental and Biological Engineering SP - 32 EP - 38 PB - Science Publishing Group SN - 2640-267X UR - https://doi.org/10.11648/j.jcebe.20200402.11 AB - A microbial composition study of sediments of contaminated (CWS) and uncontaminated (UWS) lentic ecosystems within Akwa Ibom State was carried out by analyzing the small-subunit rRNA genes to determine the effect of hydrocarbon contamination on its microbial composition and diversity. Analysis of the V4 region of the community DNA from both sediments revealed the presence of bacteria, archaea and microalgae. Bacterial sequences outnumbered archaea and microalgae. Abundance of Proteobacteria, Betaproteobacteria, Burholderiales, Alcaligenaceae, and Achrombacter were observed in the CWS and Actinobacteria, Actinomycetales, Bacillaceae, and Bacillus in the UWS. Crenarchaeota and Euryarchaeota were also observed to be present in both sediments. The genus Achromobacter and Bacillus dominated in the CWS and UWS, respectively. Uncultured bacterium with the accession number DQ404672.1 and AY917600.1 led at the species levels. Achromobacter sp.-AM232721.1 outnumbered the other species in the CWS such as Kitasatospora sp.-AF131379.1, Mycobacterium celatum-AF547908.1, Paenibacillus phyllosphaerae-NR_043008.1, Cystobacter fuscus-M94276.1, Planosporangium flavigriseum-NR_042508.1, etc. In the UWS, the dominant species was Bacillus sp.-AJ316313.1. Microalgae, Chlorella sp. and Chlorella vulgaris were also detected in both ecosystems. Diverse and distinct diversity of bacteria, archaea and microalgae are present in the sediments and only a few of them have cultured counterpart. The variation in the microbial communities from the two sites has revealed the impact of contaminants especially hydrocarbons on the microbial diversity in lentic ecosystems. VL - 4 IS - 2 ER -
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